Medić-Sarić Marica, Rastija Vesna, Bojić Mirza
University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, A. Kovacića 1, 10000 Zagreb, Croatia.
J AOAC Int. 2011 Jan-Feb;94(1):32-42.
Wine and propolis are among the richest sources of polyphenolics. Flavonoids and phenolic acids are main pharmacological components whose benefits have been shown in numerous reports. This paper presents a review of the latest applications of HPLC for determination of polyphenolics in propolis and wine. Special attention is focused on sample preparation, chromatographic conditions, and detection of polyphenolics. HPLC will undoubtedly be the most used method that is readily available for analysis, as well as for isolation of polyphenolics in complex mixtures.
葡萄酒和蜂胶是多酚类物质最丰富的来源之一。黄酮类化合物和酚酸是主要的药理成分，其益处已在众多报告中得到证实。本文综述了高效液相色谱法（HPLC）在蜂胶和葡萄酒中多酚类物质测定方面的最新应用。特别关注样品制备、色谱条件以及多酚类物质的检测。毫无疑问，HPLC将是最常用的方法，它既易于用于分析，也可用于复杂混合物中多酚类物质的分离。
