D'Mello Priscilla M, Joshi Urmila J, Shetgiri Preetam P, Dasgupta Tarun K, Darji Kamil K
Principal K.M. Kundnani College of Pharmacy, Jote Joy Bldg, Rambhau Salgaokar Marg, Cuffe Parade, Colaba, Mumbai 400 005, Maharashtra, India.
J AOAC Int. 2011 Jan-Feb;94(1):100-5.
A reversed-phase HPLC method with UV detection was developed for the determination of quercetin. The method produced linear response over a wide concentration range, with an average accuracy of 95% and average intra- and interday variation of 0.75 and 0.3, respectively. The exactness of the method was proven by determining the recovery rates from 50 to 150% of standard concentration, which were found within the acceptable range of 95 to 105%. The method was used for quantitation of quercetin in the extracts of Psidium guajava, Vitis vinifera, and extracts rich in quercetin and other flavonols in the flavonoid family.
建立了一种采用紫外检测的反相高效液相色谱法测定槲皮素。该方法在较宽的浓度范围内呈线性响应，平均准确度为95%，日内和日间平均变异系数分别为0.75和0.3。通过测定标准浓度50%至150%的回收率来证明该方法的准确性，回收率在95%至105%的可接受范围内。该方法用于番石榴、葡萄提取物以及富含槲皮素和黄酮类家族中其他黄酮醇的提取物中槲皮素的定量分析。
