Haghazali Mehrdad, Molaei Mahsa, Mashayekhi Reza, Zojaji Homayoun, Pourhoseingholi Mohammad Amin, Shooshtarizadeh Tina, Mirsattari Dariush, Zali Mohammad Reza
Department of Pathology and Gastroenterology, Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University, M.C., Tehran, Iran.
Indian J Pathol Microbiol. 2011 Jan-Mar;54(1):103-6. doi: 10.4103/0377-4929.77343.
Helicobacter pylori infect more than half of the global population. It is suggested to be related with gastritis, peptic ulcer disease (PUD), and gastric cancer.
The aim of this present study was to evaluate proinflammatory cytokines including interleukin 1, 6, 8, 10, and thrombomodulin in H. pylori-infected patients with PUD and gastric cancer.
This cross-sectional study was conducted in Taleghani Hospital on 111 patients with H. pylori infection.
Patients were divided into three groups of PUD, cancer, and control (normal on endoscopy), according to the results of endoscopy. The serum levels of interleukins 1, 6, 8, and 10 and thrombomodulin was determined using enzyme-linked immunosorbent assay (ELISA) technique. H. pylori infection was diagnosed by histological examination of the endoscopic biopsy.
One hundred eleven patients were included in the study; 30 as PUD group, 30 as gastric cancer group, and 51 as controls. There was no significant difference between the means of IL-1 and IL-10 levels among the three groups (P = 0.744 and 0.383, respectively). IL-6, IL-8, and thrombomodulin levels were found to be statically different among the three groups (P < 0.05). The level of IL-6, IL-8, and thrombomodulin in cancer group was significantly higher than PUD and control groups (P < 0.05).
There is a significant association between H. pylori infection and serum IL-6, IL-8, and thrombomodulin but such relation is not present between H. pylori and IL-1 and IL-10. Immunity response (IL-6, IL-8 and thrombomodulin) is more severe in cancer patient than PUD.
幽门螺杆菌感染全球超过半数人口。它被认为与胃炎、消化性溃疡病（PUD）和胃癌有关。
本研究的目的是评估幽门螺杆菌感染的PUD患者和胃癌患者体内的促炎细胞因子，包括白细胞介素1、6、8、10和血栓调节蛋白。
本横断面研究在塔莱加尼医院对111例幽门螺杆菌感染患者进行。
根据内镜检查结果，将患者分为PUD组、癌症组和对照组（内镜检查正常）。采用酶联免疫吸附测定（ELISA）技术测定白细胞介素1、6、8、10和血栓调节蛋白的血清水平。通过内镜活检组织学检查诊断幽门螺杆菌感染。
111例患者纳入研究；30例为PUD组，30例为胃癌组，51例为对照组。三组间IL-1和IL-10水平均值无显著差异（P分别为0.744和0.383）。发现三组间IL-6、IL-8和血栓调节蛋白水平存在统计学差异（P<0.05）。癌症组的IL-6、IL-8和血栓调节蛋白水平显著高于PUD组和对照组（P<0.05）。
幽门螺杆菌感染与血清IL-6、IL-8和血栓调节蛋白之间存在显著关联，但幽门螺杆菌与IL-1和IL-10之间不存在这种关系。癌症患者的免疫反应（IL-6、IL-8和血栓调节蛋白）比PUD患者更严重。
