Laboratory of Pharmaceutical Technology, Federal University of Parana, Curitiba, Brazil.
J Cardiovasc Pharmacol. 2011 Jun;57(6):696-701. doi: 10.1097/FJC.0b013e318217db1f.
The aim of this study was to investigate the mechanisms underlying the vasorelaxant effect induced by the polyphenolic compounds found in red wine from Vale do São Francisco. In phenylephrine (10 μM) precontracted mesenteric artery rings, the red wine caused a concentration-dependent relaxation (maximum response to phenylephrine 10 μM = 87.5% ± 6.5%, n = 10). After endothelium removal, the vasorelaxant effect elicited by red wine was attenuated (28.4% ± 4.9%, n = 10). In addition, the vasorelaxant effect induced by red wine in rings pretreated with 100 μM of N(w)-nitro-l-arginine methyl ester and 10 μM of 1H-[1,2,4] oxadiazolo-[4,3-a]-quinoxalin-1-one was attenuated (23.4% ± 5.1%, n = 7 and 11.8% ± 2.7%, n = 6, respectively). Pretreatment with atropine did not affect the vasorelaxant effect induced by red wine (81% ± 3.9%, n = 6). Furthermore, in rabbit aortic endothelial cell line, red wine 100 and 300 μg/mL caused concentration-dependent increases in nitric oxide levels (58 ± 1; 82 ± 7.9; Δ% of fluorescence, n = 5, respectively). In conclusion, we suggest that the alcohol free-lyophilized red wine induces an endothelium-dependent vasorelaxant effect due, at least in part, to a secondary increase in the concentration of nitric oxide and that this effect might be associated with phenolic compounds found in the red wine.
本研究旨在探讨产自圣弗朗西斯科谷的红葡萄酒中所含多酚化合物引起血管舒张作用的机制。在预先用苯肾上腺素（10 μM）收缩的肠系膜动脉环中，红葡萄酒引起浓度依赖性舒张（对苯肾上腺素 10 μM 的最大反应为 87.5%±6.5%，n=10）。内皮细胞去除后，红葡萄酒引起的血管舒张作用减弱（28.4%±4.9%，n=10）。此外，用 100 μM N(w)-硝基-l-精氨酸甲酯和 10 μM 1H-[1,2,4]恶二唑-[4,3-a]-喹喔啉-1-酮预处理的血管环中，红葡萄酒引起的血管舒张作用减弱（分别为 23.4%±5.1%，n=7 和 11.8%±2.7%，n=6）。阿托品预处理不影响红葡萄酒引起的血管舒张作用（81%±3.9%，n=6）。此外，在兔主动脉内皮细胞系中，红葡萄酒 100 和 300 μg/mL 引起浓度依赖性一氧化氮水平增加（58±1；82±7.9；荧光百分比变化，n=5，分别）。总之，我们认为无酒精冻干红葡萄酒引起内皮依赖性血管舒张作用，至少部分是由于一氧化氮浓度的二次增加，并且这种作用可能与红葡萄酒中存在的酚类化合物有关。
