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从出生到成熟过程中 Karagouniko 乳用绵羊脂肪组织的细胞结构和酶活性。

Cellularity and enzymatic activity of adipose tissue in the Karagouniko dairy breed of sheep from birth to maturity.

机构信息

Department of Animal Breeding and Husbandry, Agricultural University of Athens, Votanikos, Greece.

出版信息

J Anim Breed Genet. 1997 Jan 12;114(1-6):385-96. doi: 10.1111/j.1439-0388.1997.tb00524.x.

DOI:10.1111/j.1439-0388.1997.tb00524.x
PMID:21395833
Abstract

The cellular and enzymatic characteristics of dissected subcutaneous and perirenal adipose tissues were examined in a total of 102 male and female animals from commerical flocks of the Karagouniko dairy sheep. The animals were slaughtered in groups of three males and three females at 45-day intervals from birth to 720 days of age. The following determinations were made on each animal: (1) subcutaneous and perirenal chemical fat, (2) fat cell size and number and (3) the activities of NADP-isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in subcutaneous and perirenal adipose tissues. Subcutaneous adipose tissue expanded by a combination of fat cell hypertrophy and hyperplasia during the accelerating phase of the growth curve of chemical fat, after which the fat expansion was accomplished primarily by hyperplasia. The changes in perirenal chemical fat weight with increasing age were primarily due to hypertrophy of fat cells during the accelerating phase of the curve of chemical fat growth and due to a combination of hypertrophy and hyperplasia during the diminishing phase of the curve. The enzyme activities were higher in subcutaneous than in perirenal adipose tissue, with the NADP-isocitrate dehydrogenase being the most active enzyme in both tissues and sexes. The changes in enzyme activities in subcutaneous adipose tissue with increasing age were parallel to the changes in the rate of chemical fat weight during growth. ZUSAMMENFASSUNG: Zellularitaet und enzymatische Aktivitaet des Fettgewebes beim Karagouniko Milchschaf von der Geburt bis zur Reife. Es wurden die Anzahl und die Groesse der Fettzellen sowie die Aktivitaeten der NADP-Isocitrat-Dehydrogenase und der 6-Phosphogluconat Dehydrogenase in subcutanen und perirenalen Fettgewebe des Schafes waehrend des Wachstums undersucht. Das Tiermaterial bestand aus 51 maennlichen und 51 weiblichen Schafen der Karogouniko Milchrasse. Die Schafe wurden in Gruppen bestehend aus 3 maennlichen und 3 weiblichen Tieren in 45-taegigen Abstaenden von der Geburt bis zum Alter von 720 Tagen geschlachtet. Es wurden die folgenden Merkmale erfasst: Chemisches Fett, Anzahl and Groesse der Fettzellen, Aktivitaeten der gennanten Enzyme. Das subcutane Fettgewebe wuchs durch eine Kombination von Hypertrophie und Hyperplasia waehrend der beschleunigten Phase der Wachstumskurve fuer das chemische Fett. Nach dieser Wachstumsphase, das weitere Wachstum des subcutanen Fettgewebes kam vorwiegend durch Hyperplasia der Fettzellen zustande. Das perirenale Fettgewebe wuchs zunaechst durch Hypertrophie der Fettzellen und anschliessend durch eine Kombinationn von Hypertrophie und Hyperplasia. Die Aktivitaet der NADP-Isocitrat-Dehydrogenase war hoeher als die Aktivitaet der 6-Phosphogluconat-Dehydronase in beiden Geweben und Geschlechtern waehrend der gesamten Versuchsdauer. Die Aenderungen der Enzymaktivitaet waehrend des Wachstums stimmten gut ueberein mit den entsprechenden Aenderungen der taeglichen Wachstumstrate der subcutanen Fettgewebes.

摘要

对商业养殖的卡拉戈尼科奶绵羊的 102 只雄性和雌性动物的皮下和肾周脂肪组织进行了细胞和酶特性分析。这些动物在出生后至 720 天的 45 天间隔内,以每组 3 只雄性和 3 只雌性的方式进行屠宰。对每只动物进行了以下测定:(1)皮下和肾周化学脂肪,(2)脂肪细胞大小和数量,(3)皮下和肾周脂肪组织中 NADP-异柠檬酸脱氢酶和 6-磷酸葡萄糖酸脱氢酶的活性。皮下脂肪组织在化学脂肪生长曲线的加速阶段通过脂肪细胞肥大和增生的结合而扩张,此后脂肪的扩张主要通过增生来实现。肾周化学脂肪重量随年龄的增长而变化,主要是由于在化学脂肪生长曲线的加速阶段脂肪细胞肥大,以及在曲线的下降阶段肥大和增生的结合。与肾周脂肪组织相比,皮下脂肪组织中的酶活性更高,NADP-异柠檬酸脱氢酶是两种组织和性别中最活跃的酶。随着年龄的增长,皮下脂肪组织中酶活性的变化与生长过程中化学脂肪重量的变化呈平行关系。

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