Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil 4365, 21045-900, Rio de Janeiro, Brazil.
Diagn Microbiol Infect Dis. 2011 Jun;70(2):274-7. doi: 10.1016/j.diagmicrobio.2011.01.006. Epub 2011 Mar 11.
The aim of this study was to investigate the genetic relatedness of 57 KPC-2-producing Klebsiella pneumoniae isolates from 5 states in Brazil, during 2006-2009. Pulse-field gel electrophoresis analysis identified 10 pulsotypes. The pulsotype designated as Kp-RJ (Klebsiella pneumoniae-Rio de Janeiro) was the dominant clone found in the states of Rio de Janeiro and Espírito Santo. Multilocus sequence typing of Kp-RJ assigned it to ST 437. Sequence types ST11, ST16, ST25, ST70, ST101, ST105, ST423, ST442, and ST443 were also identified. These results indicate the dissemination of a successful KPC-producing clone (ST437) in Brazil, which is a single locus variant of ST258.
本研究旨在调查 2006-2009 年间巴西 5 个州的 57 株产 KPC-2 肺炎克雷伯菌的遗传相关性。脉冲场凝胶电泳分析鉴定出 10 种脉冲型。被指定为 Kp-RJ（里约热内卢肺炎克雷伯菌）的脉冲型是在里约热内卢和圣埃斯皮里图州发现的主要克隆。Kp-RJ 的多位点序列分型将其分配到 ST437。还鉴定出 ST11、ST16、ST25、ST70、ST101、ST105、ST423、ST442 和 ST443 等序列类型。这些结果表明，在巴西，一种成功的产 KPC 克隆（ST437）正在传播，它是 ST258 的单一位点变异体。
