UPMC Eye Center, Eye and Ear Institute, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
Invest Ophthalmol Vis Sci. 2010 Dec;51(12):6519-23. doi: 10.1167/iovs.10-5662. Epub 2010 Jun 23.
To test the reproducibility of spectral-domain optical coherence tomography (SD-OCT) total retinal thickness (TRT) measurements in mice.
C57Bl/6 mice were anesthetized, and three repeated volumetric images were acquired in both eyes with SD-OCT (250 A-scans × 250 frames × 1024 samplings), centered on the optic nerve head (ONH). The mice were repositioned between scans. TRT was automatically measured within a sampling band of retinal thickness with radii of 55 to 70 pixels, centered on the ONH by using custom segmentation software. The first volumetric image acquired in a given eye was used to register the remaining two SD-OCT images by manually aligning the en face images with respect to rotation and linear translation. Linear mixed-effects models were fitted to global and quadrant thicknesses, taking into account the clustering between eyes, to assess imprecision (measurement reproducibility).
Twenty-six eyes of 13 adult mice (age 13 weeks) were imaged. The mean global TRT across all eyes was 298.21 μm, with a mouse heterogeneity standard deviation (SD) of 4.88 μm (coefficient of variation [CV] = 0.016), an eye SD of 3.32 μm (CV = 0.011), and a device-related imprecision SD of 2.33 μm (CV = 0.008). The superior quadrant had the thickest mean TRT measurement (310.38 μm) and the highest (worst) imprecision SD (3.13 μm; CV = 0.010), and the inferior quadrant had the thinnest mean TRT (291.55 μm). The quadrant with the lowest (best) imprecision SD was in the nasal one (2.06 μm; CV = 0.007).
Good reproducibility was observed for SD-OCT retinal thickness measurements in mice. SD-OCT may be useful for in vivo longitudinal studies in mice.
测试频域光相干断层扫描（SD-OCT）在小鼠中总视网膜厚度（TRT）测量的可重复性。
将 C57Bl/6 小鼠麻醉，使用 SD-OCT（250 个 A 扫描×250 个帧×1024 个采样）在视神经头（ONH）处对双眼进行三次重复的容积图像采集。在扫描之间重新定位小鼠。使用定制的分割软件，在以 ONH 为中心、半径为 55 到 70 个像素的视网膜厚度采样带内自动测量 TRT。通过手动将 EN 面图像相对于旋转和平移进行对齐，将在一只眼睛中获得的第一个容积图像用于对其余两个 SD-OCT 图像进行注册。通过考虑眼睛之间的聚类，使用线性混合效应模型来评估全局和象限厚度的不精确性（测量可重复性）。
对 13 只成年小鼠（13 周龄）的 26 只眼睛进行了成像。所有眼睛的平均全局 TRT 为 298.21μm，小鼠异质性标准偏差（SD）为 4.88μm（变异系数[CV] = 0.016），眼睛 SD 为 3.32μm（CV = 0.011），设备相关的不精确性 SD 为 2.33μm（CV = 0.008）。上象限的平均 TRT 测量值最高（310.38μm），不精确性 SD 最高（最差）（3.13μm；CV = 0.010），下象限的平均 TRT 最薄（291.55μm）。不精确性 SD 最低（最好）的象限在鼻侧（2.06μm；CV = 0.007）。
在小鼠的 SD-OCT 视网膜厚度测量中观察到良好的可重复性。SD-OCT 可能对小鼠体内纵向研究有用。
