Key Laboratory of Marine Genetics and Breeding for Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai, PR China.
Int J Syst Evol Microbiol. 2012 Jan;62(Pt 1):223-228. doi: 10.1099/ijs.0.025536-0. Epub 2011 Mar 11.
A novel Gram-negative, non-motile bacterium, designated ZXM137(T), was isolated from seawater collected from a coastal region of Qingdao, China, during a massive green algae (Enteromorpha prolifera) bloom. Strain ZXM137(T) was strictly aerobic and did not accumulate poly-β-hydroxybutyrate. Growth occurred with 0.5-11.0% (w/v) NaCl, at pH 6-9 (optimum of pH 7) and at 4-45 °C (optimum at 28 °C). It contained Q-10 as the predominant ubiquinone and the major polar lipids were phosphatidylglycerol, phospholipids, and an unidentified aminolipid and lipid. The major cellular fatty acids of strain ZXM137(T) were C(18:1)ω7c, C(18:1)ω6c and 11-methyl C(18:1)ω7c. The DNA G+C content was 60.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZXM137(T) formed a distinct evolutionary lineage within the Roseobacter group in the class Alphaproteobacteria. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain ZXM137(T) represents a novel species in a new genus, for which the name Huaishuia halophila gen. nov., sp. nov. is proposed; the type strain is ZXM137(T) (=CGMCC 1.8891(T)=LMG 24854(T)).
一株新的革兰氏阴性、非运动性细菌，命名为 ZXM137(T)，从中国青岛沿海地区的海水中分离出来，当时那里发生了大规模的绿藻（浒苔）水华。菌株 ZXM137(T)是严格需氧的，不积累聚-β-羟基丁酸。生长在 0.5-11.0%（w/v）NaCl、pH6-9（最佳 pH7）和 4-45°C（最佳 28°C）的条件下。它含有 Q-10 作为主要的泛醌，主要的极性脂类是磷脂酰甘油、磷脂和一种未鉴定的胺脂和脂类。菌株 ZXM137(T)的主要细胞脂肪酸是 C(18:1)ω7c、C(18:1)ω6c 和 11-甲基 C(18:1)ω7c。DNA G+C 含量为 60.1 mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 ZXM137(T)在α变形菌纲的玫瑰杆菌群中形成了一个独特的进化谱系。根据表型、化学分类和系统发育证据，菌株 ZXM137(T)代表了一个新属中的一个新种，建议将其命名为 Huaishuia halophila 属，种，新种；模式菌株是 ZXM137(T)=CGMCC 1.8891(T)=LMG 24854(T)。
