College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1042-1047. doi: 10.1099/ijs.0.029868-0. Epub 2011 Jun 17.
Two novel Gram-stain-negative, aerobic strains (ZXM100(T) and ZXM098) were isolated from seawater in the coastal region of Qingdao (36.027° N 120.184° E), China, during a massive green algae bloom. Cells were ovoid to irregular short rods and lacked flagella. Poly-β-hydroxybutyrate was accumulated. Cells did not contain bacteriochlorophyll a. The isolates grew at NaCl concentrations of 3-9 % and 22-28 °C. C(18 : 1)ω7c, C(18 : 0) and C(16 : 0) were the major fatty acid components. The polar lipids of ZXM100(T) were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, a single lipid and an unidentified aminolipid. The predominant isoprenoid quinone of ZXM100(T) was Q-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains belonged to the family Rhodobacteraceae. Based on the results obtained in this study, strain ZXM100(T) was considered to represent a novel species in a new genus, for which the name Lentibacter algarum gen. nov., sp. nov. is proposed. The type strain is ZXM100(T) ( = LMG 24861(T) = CGMCC 1.10234(T)); the DNA G+C content of the type strain is 54.6 mol%.
两株新的革兰氏阴性、需氧菌株（ZXM100(T)和 ZXM098）是从中国青岛沿海地区（36.027° N 120.184° E）的海水中在大规模绿藻水华期间分离得到的。细胞呈卵形到不规则的短杆状，没有鞭毛。聚-β-羟基丁酸积累。细胞不含有细菌叶绿素 a。这些分离株在 3-9%的 NaCl 浓度和 22-28°C 的温度下生长。C(18 : 1)ω7c、C(18 : 0)和 C(16 : 0)是主要的脂肪酸成分。ZXM100(T)的极性脂类是磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、单一脂类和一种未鉴定的胺脂类。ZXM100(T)的主要类异戊二烯醌是 Q-10。基于 16S rRNA 基因序列的系统发育分析表明，这些菌株属于红杆菌科。根据本研究的结果，菌株 ZXM100(T)被认为代表了一个新属中的一个新种，因此提议建立一个新属 Lentibacter algarum gen. nov.，sp. nov.。模式株是 ZXM100(T)（ = LMG 24861(T) = CGMCC 1.10234(T)）；模式株的 DNA G+C 含量为 54.6 mol%。
