Department of Growth and Differentiation of Cell Populations, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 2011;60(3):403-17. doi: 10.33549/physiolres.932045. Epub 2011 Mar 14.
This review briefly outlines the history and possibilities of bone reconstruction using various types of artificial materials, which allow interaction with cells only on the surface of the implant or enable ingrowth of cells inside the material. Information is also provided on the most important properties of bone cells taking part in bone tissue development, and on diseases and regeneration. The most common cell types used for testing cell-material interaction in vitro are listed, and the most commonly used approaches to this testing are also mentioned. A considerable part of this review is dedicated to the physical and chemical properties of the material surface, which are decisive for the cell-material interaction, and also to modifications to the surface of the material aimed at integrating it better with the surrounding bone tissue. Special attention is paid to the effects of nanoscale and microscale surface roughness on cell behaviour, to material surface patterning, which allows regionally-selective adhesion and growth of cells, and also to the surface chemistry. In addition, coating the materials with bioactive layers is examined, particularly those created by deposition of fullerenes, hybrid metal-fullerene composites, carbon nanotubes, nanocrystalline diamond films, diamond-like carbon, and nanocomposite hydrocarbon plasma polymer films enriched with metals.
这篇综述简要概述了使用各种类型的人工材料进行骨重建的历史和可能性，这些材料仅允许在植入物表面与细胞相互作用，或者允许细胞在材料内部生长。还提供了参与骨组织发育的骨细胞的最重要特性的信息，以及疾病和再生的信息。列出了用于体外测试细胞-材料相互作用的最常见的细胞类型，并提到了最常用的测试方法。本综述的相当一部分专门介绍了材料表面的物理和化学性质，这些性质对细胞-材料相互作用至关重要，还介绍了旨在更好地将材料与周围骨组织整合的材料表面改性。特别关注纳米级和微观级表面粗糙度对细胞行为的影响、材料表面图案化，这允许细胞的区域选择性附着和生长，以及表面化学。此外，还研究了材料的涂层，特别是那些由富勒烯沉积、混合金属富勒烯复合材料、碳纳米管、纳米晶金刚石膜、类金刚石碳和富含金属的纳米复合碳氢等离子体聚合物膜形成的涂层。
