Department of Infectious Diseases, Osaka Prefectural Institute of Public Health.
Jpn J Infect Dis. 2013;66(5):436-8. doi: 10.7883/yoken.66.436.
Isolation of novel types of human adenovirus D (HAdV-53, -54, and -56) from keratoconjunctivitis patients has been reported since 2008. We examined the molecular epidemiology of HAdV-D strains using 39 field isolates collected from epidemic keratoconjunctivitis (EKC) patients from 2001 to 2010 in the province of Osaka, Japan. The molecular types were analyzed by sequencing partial penton base gene, loop 1 in the hexon, and complete fiber genes. Of the 39 isolates, the majority were HAdV-19 (14/39, 35.9%) and -37 (13/39, 33.3%), followed by -53 (4/39, 10.3%) and -54 (8/39, 20.5%). Analysis of annual distribution showed that HAdV-19 and -37 were detected before 2004, whereas HAdV-53 and -54 were first identified in 2001 and 2003, respectively, and persistently detected during the study period. It is noted that both HAdV-53 and -54 isolates were misclassified by the serological method. Altogether, the molecular analysis elucidated the epidemiology of HAdV-D and presence of novel types from the early 2000s in Osaka. Further genetic analysis of serologically classified HAdV-D isolates may provide insights into the epidemiology of EKC.
自 2008 年以来，已从角膜炎结膜炎患者中分离出新型人腺病毒 D（HAdV-53、-54 和-56）。我们使用从日本大阪省 2001 年至 2010 年流行角膜炎结膜炎（EKC）患者中采集的 39 个流行株，检测了 HAdV-D 株的分子流行病学。通过测序部分五邻体基底基因、六邻体环 1 和完整纤维基因分析分子类型。在 39 个分离株中，大多数是 HAdV-19（14/39，35.9%）和-37（13/39，33.3%），其次是-53（4/39，10.3%）和-54（8/39，20.5%）。年度分布分析表明，HAdV-19 和-37 于 2004 年前被检测到，而 HAdV-53 和-54 分别于 2001 年和 2003 年首次被发现，并在研究期间持续被检测到。值得注意的是，两种 HAdV-53 和-54 分离株均被血清学方法错误分类。总的来说，分子分析阐明了 21 世纪初大阪的 HAdV-D 流行病学和新型病毒的存在。对血清学分型的 HAdV-D 分离株进行进一步的遗传分析可能有助于了解 EKC 的流行病学。
