Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Hong Kong, China.
Opt Lett. 2011 Mar 15;36(6):834-6. doi: 10.1364/OL.36.000834.
Direct visualization of microvasculature provides significant insights in microcirculation and critically impacts the diagnosis and treatment of microcirculatory diseases. Recently, we discovered that the high-energy Soret fluorescence of hemoglobin peaked at 438 nm with an extremely short lifetime becomes strongly visible under two-photon excitation. Based on the distinct spectral and temporal characteristics of hemoglobin fluorescence, we demonstrated that two-photon fluorescence microscopy could become a powerful tool for label-free in vivo imaging of microvasculature in tissue.
直接观察微血管可深入了解微循环，并对微血管疾病的诊断和治疗产生重大影响。最近，我们发现血红蛋白的高能 Soret 荧光在 438nm 处具有峰值，且其寿命极短，在双光子激发下会变得非常明显。基于血红蛋白荧光的独特光谱和时间特性，我们证明了双光子荧光显微镜可以成为一种强大的无标记活体组织微血管成像工具。
