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红正蚓蛋白提取物DLBS1033具有抗血栓和溶栓活性。

DLBS1033, a protein extract from Lumbricus rubellus, possesses antithrombotic and thrombolytic activities.

作者信息

Trisina Jessica, Sunardi Febrina, Suhartono Maggy T, Tjandrawinata Raymond R

机构信息

Division of Protein Biochemistry and Molecular Pharmacology, Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V Block PP no. 7, Kawasan Industri Jababeka II, Cikarang 17550, Indonesia.

出版信息

J Biomed Biotechnol. 2011;2011:519652. doi: 10.1155/2011/519652. Epub 2011 Mar 3.

DOI:10.1155/2011/519652
PMID:21403877
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3051164/
Abstract

The medicinal value of earthworm has been widely known since the history of Asian ancient medicine. This present study aims to determine the mechanism of action and effect of a standardized extract of Lumbricus rubellus named as DLBS1033. The fibrinogen degradation, antiplatelet aggregation, and ex vivo antithrombotic assay using human blood were performed to study antithrombotic activity. Fibrin plate and clot lysis assay were also done to examine thrombolytic properties. DLBS1033 was found to possess fibrinogenolytic activity on α-, β-, and γ-chain of fibrinogen. It also induced antiplatelet aggregation and prolonged blood clotting time, which further confirmed its antithrombotic properties. In addition, thrombolytic properties of DLBS1033 were shown with its fast and long-acting fibrinolytic activity, as well as its effective blood clot lysis activities. In conclusion, DLBS1033 conferred antithrombotic and thrombolytic action which could be used as a safe and promising oral thrombolytic drug.

摘要

自亚洲古代医学史以来，蚯蚓的药用价值就已广为人知。本研究旨在确定一种名为DLBS1033的赤子爱胜蚓标准化提取物的作用机制和效果。采用纤维蛋白原降解、抗血小板聚集和用人血进行的体外抗血栓试验来研究抗血栓活性。还进行了纤维蛋白平板和血凝块溶解试验以检测溶栓特性。发现DLBS1033对纤维蛋白原的α-、β-和γ链具有纤维蛋白原溶解活性。它还诱导抗血小板聚集并延长血液凝固时间，这进一步证实了其抗血栓特性。此外，DLBS1033的溶栓特性表现为其快速且长效的纤维蛋白溶解活性以及有效的血凝块溶解活性。总之，DLBS1033具有抗血栓和溶栓作用，可作为一种安全且有前景的口服溶栓药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/8dac4b3c489f/JBB2011-519652.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/a83597b46848/JBB2011-519652.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/3e83a3fde1da/JBB2011-519652.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/367bb872aa60/JBB2011-519652.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/bedd3f902e9c/JBB2011-519652.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/01d91edd4762/JBB2011-519652.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/8dac4b3c489f/JBB2011-519652.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/a83597b46848/JBB2011-519652.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/50129780da1a/JBB2011-519652.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/dd163e78b13f/JBB2011-519652.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/5deffd97b298/JBB2011-519652.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/aed572e9b8bf/JBB2011-519652.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/3e83a3fde1da/JBB2011-519652.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/367bb872aa60/JBB2011-519652.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/bedd3f902e9c/JBB2011-519652.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/01d91edd4762/JBB2011-519652.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d62/3051164/8dac4b3c489f/JBB2011-519652.010.jpg

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