Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan School of Dentistry, 24 Frank Lloyd Wright Dr., Lobby M, Box 422, Ann Arbor, MI 48106 USA.
J Dent Res. 2011 Jun;90(6):752-8. doi: 10.1177/0022034511399908. Epub 2011 Mar 15.
The purpose of this study was to determine the role of saliva-derived biomarkers and periodontal pathogens during periodontal disease progression (PDP). One hundred human participants were recruited into a 12-month investigation. They were seen bi-monthly for saliva and clinical measures and bi-annually for subtraction radiography, serum and plaque biofilm assessments. Saliva and serum were analyzed with protein arrays for 14 pro-inflammatory and bone turnover markers, while qPCR was used for detection of biofilm. A hierarchical clustering algorithm was used to group study participants based on clinical, microbiological, salivary/serum biomarkers, and PDP. Eighty-three individuals completed the six-month monitoring phase, with 39 [corrected] exhibiting PDP, while 44 [corrected] demonstrated stability. Participants assembled into three clusters based on periodontal pathogens, serum and salivary biomarkers. Cluster 1 members displayed high salivary biomarkers and biofilm; 71% [corrected] of these individuals were undergoing PDP. Cluster 2 members displayed low biofilm and biomarker levels; 76% [corrected] of these individuals were stable. Cluster 3 members were not discriminated by PDP status; however, cluster stratification followed groups 1 and 2 based on thresholds of salivary biomarkers and biofilm pathogens. The association of cluster membership to PDP was highly significant (p < 0.0007). [corrected] The use of salivary and biofilm biomarkers offers potential for the identification of PDP or stability (ClinicalTrials.gov number, CT00277745).
本研究旨在确定唾液生物标志物和牙周病病原体在牙周病进展（PDP）过程中的作用。招募了 100 名人类参与者进行为期 12 个月的研究。他们每两个月接受一次唾液和临床检查，每两年接受一次减影放射学、血清和牙菌斑生物膜评估。使用蛋白质阵列分析唾液和血清中的 14 种促炎和骨转换标志物，同时使用 qPCR 检测生物膜。使用层次聚类算法根据临床、微生物学、唾液/血清生物标志物和 PDP 将研究参与者分为不同的组。83 名参与者完成了六个月的监测阶段，其中 39 名[校正]出现了 PDP，而 44 名[校正]表现出稳定。根据牙周病病原体、血清和唾液生物标志物，参与者被分为三个聚类。聚类 1 成员显示出高唾液生物标志物和生物膜；其中 71%[校正]的个体正在经历 PDP。聚类 2 成员显示出低生物膜和生物标志物水平；其中 76%[校正]的个体稳定。聚类 3 成员不能通过 PDP 状态进行区分；然而，聚类分层根据唾液生物标志物和生物膜病原体的阈值遵循 1 类和 2 类。聚类成员与 PDP 的关联具有高度显著性（p < 0.0007）。[校正]唾液和生物膜生物标志物的使用为识别 PDP 或稳定性提供了潜力（临床试验编号，CT00277745）。
