MTCC-Microbial Type Culture Collection & Gene Bank, Institute of Microbial Technology, a Constituent Laboratory of Council of Scientific and Industrial Research, Government of India, Chandigarh, India.
Antonie Van Leeuwenhoek. 2011 Jun;100(1):129-35. doi: 10.1007/s10482-011-9573-2. Epub 2011 Mar 16.
A gram-negative, motile, straight to curved rod shaped, pink pigmented bacterium was isolated from a soil sample collected from the rhizosphere of an Indian medicinal plant, Nerium indicum (Chuvanna arali) and subjected to a detailed polyphasic taxonomic study. The strain, designated as IMTB-1969(T), matched with most of the phenotypic and chemotaxonomic properties of the genus Pontibacter and represents a novel species. The major fatty acids of the strain were monounsaturated iso/anteiso branched C17 fatty acids (45.1%) and iso-C15:0 (16.5%). MK-7 was the predominant isoprenoid quinone. According to 16S rRNA gene sequence analysis, strain IMTB-1969(T) was indicated to belonged to the phylum Bacteroidetes and further phylogenetic analysis revealed that the strain IMTB-1969(T) belongs to the family Cytophagaceae and genus Pontibacter. The highest 16S rRNA gene sequence similarity was with Pontibacter korlensis CCTCC AB 206081(T) (97.2%) and lower sequence similarity was observed with other species in the genus Pontibacter (95.9-94.0%). DNA-DNA relatedness study of the strain IMTB-1969(T) confirmed that it represents a novel species. The G+C content of the genomic DNA was 52.2 (±0.5) mol%. The results of physiological and biochemical tests allowed the genotypic and phenotypic distinction of strain IMTB-1969(T) from its closest phylogenetic relatives. The strain IMTB-1969(T) should be classified as novel species of the genus Pontibacter, for which the name Pontibacter rhizosphera sp. nov. is proposed. The type strain is IMTB-1969(T) (=MTCC 10673(T) = DSM 24399(T)).
从印度药用植物夹竹桃（Chuvanna arali）的根际土壤样本中分离到一株革兰氏阴性、运动性、直或弯曲的棒状、粉红色色素的细菌，并对其进行了详细的多相分类学研究。该菌株被命名为 IMTB-1969(T)，与大多数Pontibacter 属的表型和化学分类特性相匹配，代表了一个新的物种。该菌株的主要脂肪酸为单不饱和异/反式支链 C17 脂肪酸（45.1%）和 iso-C15:0（16.5%）。MK-7 是主要的异戊二烯醌。根据 16S rRNA 基因序列分析，菌株 IMTB-1969(T)属于 Bacteroidetes 门，进一步的系统发育分析表明，该菌株 IMTB-1969(T)属于 Cytophagaceae 科和 Pontibacter 属。与菌株 IMTB-1969(T)最相似的 16S rRNA 基因序列来自 Pontibacter korlensis CCTCC AB 206081(T)（97.2%），与该属其他种的序列相似性较低（95.9-94.0%）。菌株 IMTB-1969(T)的 DNA-DNA 同源性研究证实，它代表了一个新的物种。基因组 DNA 的 G+C 含量为 52.2（±0.5）mol%。生理生化试验结果表明，菌株 IMTB-1969(T)在基因型和表型上与最接近的系统发育亲缘关系的菌株不同。该菌株 IMTB-1969(T)应归类为 Pontibacter 属的新种，建议命名为 Pontibacter rhizosphera sp. nov.。其模式菌株为 IMTB-1969(T)（=MTCC 10673(T) = DSM 24399(T)）。
