State Key Laboratory of Biogeology and Environmental Geology & Department of Biological Science and Technology, School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan, China; School of Chemical and Material Engineering, Hubei Polytechnic University, Huangshi, China.
State Key Laboratory of Biogeology and Environmental Geology & Department of Biological Science and Technology, School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan, China.
PLoS One. 2014 Mar 19;9(3):e92294. doi: 10.1371/journal.pone.0092294. eCollection 2014.
Few diazotrophs have been found to belong to the family Cytophagaceae so far. In the present study, a Gram-negative, rod-shaped bacterium that forms red colonies, was isolated from sands of the Takalamakan desert. It was designated H4XT. Phylogenetic and biochemical analysis indicated that the isolate is a new species of the genus Pontibacter. The 16S rRNA gene of H4XT displays 94.2-96.8% sequence similarities to those of other strains in Pontibacter. The major respiratory quinone is menaquinone-7 (MK-7). The DNA G+C content is 46.6 mol%. The major cellular fatty acids are iso-C15∶0, C16∶1ω5c, summed feature 3 (containing C16∶1ω6c and/or C16∶1ω7c) and summed feature 4 (comprising anteiso-C17∶1B and/or iso-C17∶1I). The major polar lipids are phosphatidylethanolamine (PE), one aminophospholipid (APL) and some unknown phospholipids (PLs). It is interesting to see that this bacterium can grow very well in a nitrogen-free medium. PCR amplification suggested that the bacterium possesses at least one type of nitrogenase gene. Acetylene reduction assay showed that H4XT actually possesses nitrogen-fixing activity. Therefore, it can be concluded that H4XT is a new diazotroph. We thus referred it to as Pontibacter diazotrophicus sp. nov. The type strain is H4XT ( = CCTCC AB 2013049T = NRRL B-59974T).
迄今为止，仅有少数固氮菌被发现属于噬胞菌科。本研究从塔克拉玛干沙漠的沙中分离到一株革兰氏阴性、杆状、形成红色菌落的细菌，将其命名为 H4XT。系统发育和生化分析表明，该分离株是 Pontibacter 属的一个新种。H4XT 的 16S rRNA 基因与 Pontibacter 属的其他菌株显示出 94.2-96.8%的序列相似性。主要呼吸醌为menaquinone-7 (MK-7)。DNA G+C 含量为 46.6 mol%。主要细胞脂肪酸为 iso-C15∶0、C16∶1ω5c、特征 3 总和（包含 C16∶1ω6c 和/或 C16∶1ω7c）和特征 4 总和（包括 anteiso-C17∶1B 和/或 iso-C17∶1I）。主要极性脂为磷脂酰乙醇胺 (PE)、一种氨基磷脂 (APL) 和一些未知磷脂 (PLs)。有趣的是，这种细菌可以在无氮培养基中很好地生长。PCR 扩增表明该细菌至少拥有一种氮酶基因。乙炔还原测定表明 H4XT 实际上具有固氮活性。因此，可以得出结论，H4XT 是一种新的固氮菌。我们将其命名为 Pontibacter diazotrophicus sp. nov. 模式菌株为 H4XT（ = CCTCC AB 2013049T = NRRL B-59974T）。
Zotero 插件
