Zhuo Shuangmu, Yan Jun, Chen Gang, Chen Jianxin, Liu Yuchun, Lu Jianping, Zhu Xiaoqin, Jiang Xingshan, Xie Shusen
Biomed Opt Express. 2011 Feb 16;2(3):615-9. doi: 10.1364/BOE.2.000615.
Real-time histology or virtual biopsy for the diagnosis of colonic cancer is of great medical significance. In this work, we show that label-free multiphoton imaging is feasible and effective in monitoring colonic cancer progression by providing cellular and subcellular details in fresh, unfixed, unstained colonic specimens. Our results also demonstrate the capability of using tissue quantitative analysis of the redox ratio for quantifying colonic cancer progression. These results suggest that multiphoton microscopy has potential to become an in situ histological tool, which is free from the labeling requirement of conventional methods, for the early diagnosis and detection of malignant lesions in the colon.
用于结肠癌诊断的实时组织学或虚拟活检具有重大医学意义。在这项工作中，我们表明无标记多光子成像在监测结肠癌进展方面是可行且有效的，它能在新鲜、未固定、未染色的结肠标本中提供细胞和亚细胞细节。我们的结果还证明了利用氧化还原比率的组织定量分析来量化结肠癌进展的能力。这些结果表明，多光子显微镜有潜力成为一种原位组织学工具，它无需传统方法的标记要求，可用于结肠癌恶性病变的早期诊断和检测。
