Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
Arch Virol. 2011 Jul;156(7):1257-62. doi: 10.1007/s00705-011-0966-3. Epub 2011 Mar 23.
Phylogenetic and evolutionary patterns of the six internal genes of an equine H5N1 influenza A virus isolated in Egypt on 2009 were analyzed using direct sequencing. All of the internal genes of the equine H5N1 strain showed a genetic pattern potentially related to Eurasian lineages. Variable dendrogram topologies revealed an absence of reassortment in the equine strain while confirming its close relatedness to other Egyptian H5N1 strains from human and avian species. The equine strain is characterized by a variety of amino acid substitutions in six internal proteins compared to the available Egyptian H5N1 strains. Interestingly, the equine strain displayed amino acids in the PB2, PA, M2 and NS2 proteins that are unique among the available H5N1 sequences in the flu database, and their potential effect on virulence needs to be further investigated.
对 2009 年在埃及分离的一株马源 H5N1 流感病毒的六个内部基因进行了直接测序，分析了其系统进化和演变模式。马源 H5N1 株的所有内部基因均显示出与欧亚谱系潜在相关的遗传模式。可变的系统发生树拓扑结构表明，马源株中不存在重配现象，同时证实其与来自人类和禽类的其他埃及 H5N1 株密切相关。与现有的埃及 H5N1 株相比，马源株的六个内部蛋白中有多种氨基酸替换。有趣的是，马源株在 PB2、PA、M2 和 NS2 蛋白中显示出的氨基酸在流感数据库中现有的 H5N1 序列中是独特的，其对毒力的潜在影响需要进一步研究。
