Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-10 Nishi-5, 060-0810, Kita-ku, Sapporo, Japan.
Environ Health Prev Med. 1998 Jul;3(2):106-12. doi: 10.1007/BF02931793.
Chemical carcinogens, such as chloroform and trichloroethylene, are present in drinking water in Japan. As these contaminants are believed to have a role in carcinogenesis, we examined if chloroform and trichloroethylene, as well as methylene chloride, xylene, benzene, and ethanol, have the ability to generate hydrogen peroxide (H(2)O(2)) in human polymorphonuclear leukocytes (PMN) and human leukemia (HL-60) cells. Methylene chloride, benzene, xylene, trichloroethylene, and ethanol did not increase cellular H(2)O(2): production as measured by flow cytometry nor as observed by confocal laser microscopy. In PMN and RAuntreated HL-60 cells chloroform did not significantly affect H(2)O(2) levels. However, in HL-60 cells sensitized by pretreatment of 10 nM retinoic acid (RA) for 12 h, chloroform induced a significant increase in H(2)O(2), but the increase induced by trichloroethylene was not significant. The observed increase in fluorescence was confirmed using a confocal laser microscope. These results indicate that chloroform and trichloroethylene may stimulate H(2)O(2) production in HL60 cells sensitized by pretreatment of RA. Our method may be useful to test if weak stimulants can stimulate intracellular H(2)O(2) production.
在日本,饮用水中存在化学致癌物质,如氯仿和三氯乙烯。由于这些污染物被认为在致癌过程中起作用,我们研究了氯仿、三氯乙烯以及二氯甲烷、二甲苯、苯和乙醇是否具有在人多形核白细胞(PMN)和人白血病(HL-60)细胞中生成过氧化氢(H2O2)的能力。 流式细胞术和共聚焦激光显微镜观察均未显示二氯甲烷、苯、二甲苯、三氯乙烯和乙醇增加细胞内 H2O2 的产生。在未经 RA 预处理的 PMN 和 RAuntreated HL-60 细胞中,氯仿对 H2O2 水平没有显著影响。然而,在经 10 nM 视黄酸(RA)预处理 12 h 致敏的 HL-60 细胞中,氯仿诱导 H2O2 显著增加,但三氯乙烯诱导的增加不显著。使用共聚焦激光显微镜证实了观察到的荧光增加。这些结果表明,氯仿和三氯乙烯可能刺激 RA 预处理致敏的 HL60 细胞中 H2O2 的产生。我们的方法可能有助于测试弱刺激物是否可以刺激细胞内 H2O2 的产生。