斯氏假单胞菌OX1的甲苯/邻二甲苯单加氧酶对三氯乙烯、1,1-二氯乙烯和氯仿的氧化作用
Oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1.
作者信息
Chauhan S, Barbieri P, Wood T K
机构信息
Department of Chemical and Biochemical Engineering, University of California, Irvine, California 92697-2575, USA.
出版信息
Appl Environ Microbiol. 1998 Aug;64(8):3023-4. doi: 10.1128/AEM.64.8.3023-3024.1998.
Abstract
Toluene/o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1, which oxidizes toluene and o-xylene, was examined for its ability to degrade the environmental pollutants trichloroethylene (TCE), 1, 1-dichloroethylene (1,1-DCE), cis-1,2-DCE, trans-1,2-DCE, chloroform, dichloromethane, phenol, 2,4-dichlorophenol, 2,4,5-trichlorophenol, 2,4,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, and 2,3,4,5, 6-pentachlorophenol. Escherichia coli JM109 that expressed ToMO from genes on plasmid pBZ1260 under control of the lac promoter degraded TCE (3.3 microM), 1,1-DCE (1.25 microM), and chloroform (6.3 microM) at initial rates of 3.1, 3.6, and 1.6 nmol/(min x mg of protein), respectively. Stoichiometric amounts of chloride release were seen, indicating mineralization (2.6, 1.5, and 2.3 Cl- atoms per molecule of TCE, 1,1-DCE, and chloroform, respectively). Thus, the substrate range of ToMO is extended to include aliphatic chlorinated compounds.
摘要
对来自施氏假单胞菌OX1的甲苯/邻二甲苯单加氧酶（ToMO）进行了研究，该酶可氧化甲苯和邻二甲苯，检测其降解环境污染物三氯乙烯（TCE）、1,1 - 二氯乙烯（1,1 - DCE）、顺式 - 1,2 - 二氯乙烯、反式 - 1,2 - 二氯乙烯、氯仿、二氯甲烷、苯酚、2,4 - 二氯苯酚、2,4,5 - 三氯苯酚、2,4,6 - 三氯苯酚、2,3,5,6 - 四氯苯酚和2,3,4,5,6 - 五氯苯酚的能力。在乳糖启动子控制下，从质粒pBZ1260上的基因表达ToMO的大肠杆菌JM109分别以3.1、3.6和1.6 nmol/（min×mg蛋白质）的初始速率降解TCE（3.3 microM）、1,1 - DCE（1.25 microM）和氯仿（6.3 microM）。观察到化学计量的氯离子释放，表明矿化（每分子TCE、1,1 - DCE和氯仿分别释放2.6、1.5和2.3个氯原子）。因此，ToMO的底物范围得以扩展，包括脂肪族氯化化合物。
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