基于空间调制荧光发射的光流体检测器监测全血中的 CD4。

Monitoring CD4 in whole blood with an opto-fluidic detector based on spatially modulated fluorescence emission.

机构信息

Palo Alto Research Center, Electronic Materials and Devices Laboratory, Palo Alto, CA 94304, USA.

出版信息

Cytometry A. 2011 Apr;79(4):317-24. doi: 10.1002/cyto.a.21042.

DOI:10.1002/cyto.a.21042

Abstract

We describe a new optical detection technique, termed "spatially modulated fluorescence emission," that delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The detection technique is demonstrated with measurements of absolute CD4+ and percentage CD4 counts in human blood. Benchmarking against a commonly used commercial instrument for this test yields excellent agreement for both absolute CD4 and percentage CD4.

摘要

我们描述了一种新的光学检测技术，称为“空间调制荧光发射”，它提供了高信噪比的区分，而不需要精密光学器件，从而实现了一种可以结合高性能、鲁棒性、紧凑性、低成本和易用性的流式细胞仪。该检测技术通过对人体血液中绝对 CD4+和百分比 CD4 的测量进行了演示。与该测试中常用的商业仪器进行基准测试，对于绝对 CD4 和百分比 CD4 都有很好的一致性。