Laboratory for Biomaterials, Department of Materials Science and Engineering, Indian Institute of Technology, Kanpur 208016, Uttar Pradesh, India.
J Biomed Mater Res B Appl Biomater. 2011 Jul;98(1):18-29. doi: 10.1002/jbm.b.31827. Epub 2011 Mar 22.
From the point of view of biocompatibility of bone analog materials, cell-material interaction is of fundamental importance. In this article, we report the effect of pulse electric field stimulation on cell-material interaction by analyzing cellular functionality and viability. An in-house fabricated pulse electric field setup was used for the application of electric field during cell culture experiments. To optimize voltage/electric field, the first set of exploratory experiments was conducted with varying field strength at fixed frequency, and subsequently, the frequency of the electrical stimulation was varied to study its influence on the proliferation of L929 mouse fibroblast cells on gelatin-coated control disc. Subsequently, L929 cells were cultured on hydroxyapatite (HA) and HA-40 wt % BaTiO₃ composite. Cell-cultured samples were analyzed qualitatively as well as quantitatively using fluorescence microscope and scanning electron microscope. It has been demonstrated that due to the application of electric field during the cell culture experiment, the cell proliferation and the cell spreading on the surface of the biomaterials were enhanced within a narrow window of voltage/frequency of electrical stimulation. At lower field intensities, the energy density is quite low and increases parabolically with field strength. There is no significant increase in the temperature (ΔT ~10⁻⁵ K) of the medium due to the application of short duration pulse electric field. This led us to believe that electric field with appropriate strength and duration can enhance the cell-material interaction.
从骨类似物材料的生物相容性角度来看，细胞与材料的相互作用至关重要。在本文中，我们通过分析细胞功能和活力来报告脉冲电场刺激对细胞-材料相互作用的影响。我们使用自制的脉冲电场装置在细胞培养实验中应用电场。为了优化电压/电场，我们首先进行了一系列探索性实验，在固定频率下改变场强，随后改变电刺激的频率，以研究其对在明胶涂层对照盘中增殖的 L929 小鼠成纤维细胞的影响。随后，将 L929 细胞培养在羟基磷灰石（HA）和 HA-40wt%BaTiO3 复合材料上。使用荧光显微镜和扫描电子显微镜对细胞培养样品进行定性和定量分析。结果表明，由于在细胞培养实验中施加了电场，细胞增殖和细胞在生物材料表面的铺展在电压/电场刺激的窄窗口内得到增强。在较低的场强下，能量密度相当低，并随场强呈抛物线式增加。由于应用了短持续时间的脉冲电场，介质的温度（ΔT~10⁻⁵K）没有明显升高。这使我们相信，适当强度和持续时间的电场可以增强细胞-材料相互作用。
