Department of Biological Sciences, Indian Institute of Science Education and Research-Kolkata, Mohanpur Campus, Mohanpur - 741252, India.
J Nanobiotechnology. 2011 Mar 25;9:9. doi: 10.1186/1477-3155-9-9.
The DNA degradation potential and anti-cancer activities of copper nanoparticles of 4-5 nm size are reported. A dose dependent degradation of isolated DNA molecules by copper nanoparticles through generation of singlet oxygen was observed. Singlet oxygen scavengers such as sodium azide and Tris [hydroxyl methyl] amino methane were able to prevent the DNA degradation action of copper nanoparticles confirming the involvement of activated oxygen species in the degradation process. Additionally, it was observed that the copper nanoparticles are able to exert cytotoxic effect towards U937 and Hela cells of human histiocytic lymphoma and human cervical cancer origins, respectively by inducing apoptosis. The growth characteristics of U937 and Hela cells were studied applying various concentrations of the copper nanoparticles.
本文报道了粒径为 4-5nm 的铜纳米粒子的 DNA 降解能力和抗癌活性。通过生成单线态氧，观察到铜纳米粒子对分离的 DNA 分子的剂量依赖性降解。单线态氧清除剂，如叠氮化钠和三羟甲基氨基甲烷，能够阻止铜纳米粒子对 DNA 的降解作用，从而证实了活性氧物质在降解过程中的参与。此外，还观察到铜纳米粒子能够通过诱导细胞凋亡，对人组织细胞淋巴瘤来源的 U937 细胞和人宫颈癌来源的 Hela 细胞分别发挥细胞毒性作用。应用不同浓度的铜纳米粒子研究了 U937 和 Hela 细胞的生长特性。
