Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, USTHB, BP 32, EL ALIA, Bab Ezzouar, Algiers, Algeria.
Curr Microbiol. 2013 May;66(5):462-6. doi: 10.1007/s00284-012-0300-7. Epub 2013 Jan 10.
A novel, Gram-positive, moderately halophilic bacterium, oxidase- and catalase-positive designated FarD(T) was isolated from sediments of a saline lake located in Taghit, 93 km from Bechar, southwest of Algeria. Cells were rod-shaped, endospore forming, and motile. Growth occurred at 15-40 °C (optimum, 35 °C), pH 6.0-12.0 (optimum, 7.0) and in the presence of 1-20 % NaCl (optimum, 10 %). Strain FarD(T) used glucose, mannitol, melibiose, D-mannose, and 5 ketogluconate. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and three phospholipids; MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C15:0, anteiso C17:0, C20:0, and anteiso C19:0. The DNA G+C content was 42.1 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA gene sequence indicated that strain FarD(T) had as its closest relative Virgibacillus salinus (similarity of 96.3 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain FarD(T) is proposed as a novel species of the genus Virgibacillus within the order Clostridiales, for which the name V. natechei is proposed. The type strain is FarD(T) (=DSM 25609(T) = CCUG 62224(T)).
一种新型革兰氏阳性、中度嗜盐的细菌，氧化酶和过氧化氢酶阳性，被命名为 FarD(T)，是从位于阿尔及利亚西南部贝沙尔 93 公里的塔吉特的一个盐湖沉积物中分离出来的。细胞呈杆状，形成芽孢，可运动。生长在 15-40°C（最佳温度为 35°C）、pH 值为 6.0-12.0（最佳值为 7.0）和 1-20%NaCl（最佳值为 10%）的环境中。菌株 FarD(T) 使用葡萄糖、甘露醇、蜜二糖、D-甘露糖和 5 酮葡萄糖酸。极性脂包括双磷脂酰甘油、磷脂酰甘油和三种磷脂；MK-7 是主要的menaquinone。主要的细胞脂肪酸是anteiso C15:0、anteiso C17:0、C20:0 和 anteiso C19:0。DNA G+C 含量为 42.1 mol%。小亚基核糖体 RNA 基因序列的系统发育分析表明，菌株 FarD(T)与Virgibacillus salinus（相似性为 96.3%）最为接近。基于表型、系统发育和分类学特征，菌株 FarD(T)被提议为梭菌目Clostridiales 属 Virgibacillus 的一个新种，建议将其命名为 V. natechei。模式菌株为 FarD(T)=DSM 25609(T)=CCUG 62224(T)。
