Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA.
Cold Spring Harb Perspect Biol. 2012 Mar 1;4(3):a003582. doi: 10.1101/cshperspect.a003582.
Aptamers and the SELEX process were discovered over two decades ago. These discoveries have spawned a productive academic and commercial industry. The collective results provide insights into biology, past and present, through an in vitro evolutionary exploration of the nature of nucleic acids and their potential roles in ancient life. Aptamers have helped usher in an RNA renaissance. Here we explore some of the evolution of the aptamer field and the insights it has provided for conceptualizing an RNA world, from its nascence to our current endeavor employing aptamers in human proteomics to discover biomarkers of health and disease.
适体和 SELEX 技术是二十多年前发现的。这些发现催生了一个富有成效的学术和商业产业。通过对核酸的本质及其在古代生命中的潜在作用的体外进化探索，这些发现为我们提供了对过去和现在生物学的深入了解。适体帮助迎来了 RNA 的复兴时代。在这里，我们探讨了适体领域的一些发展，以及它为我们理解 RNA 世界提供的启示，从它的起源到我们目前利用适体进行人类蛋白质组学研究以发现健康和疾病生物标志物的努力。
