Faculté des Sciences Biologiques et des Sciences Agronomiques, Université Mouloud MAMMERI BP 17 Tizi-Ouzou, Algeria.
Molecules. 2011 Mar 22;16(3):2646-57. doi: 10.3390/molecules16032646.
Three different altitudes were selected to study the variability of terpenoid composition from leaves of female plants of Pistacia lentiscus L. throughout the elevation gradient. GC-MS analyses showed that terpenoid contents change with altitude. Forty nine compounds were identified with a high interpopulation variability for low- and midaltitude sites that also exhibited the same major components when data were expressed on dry weight basis. However, Two-Way-ANOVA followed by Tukey's post hoc test showed that monoterpene hydrocarbons increased with elevation, giving values of 21.7, 37.5 and 221.5 µg g⁻¹ dw for low- mid- and highlands, respectively. On the other hand, applying P.C.A. with data expressed in percentage of the chromatogram of the volatile extract led to the identification of three chemotypes associated with altitudinal levels. In highlands (Group I), the major compounds were β-caryophyllene (12%), δ-cadinene (9.3%) and a-pinene (6.3%) while in midlands (Group II), β-caryophyllene (11.5%), δ-cadinene (8.6%) and caryophyllene oxide (6.8%) were the main components. In lowlands (Group III) δ-cadinene (10.9%), cubebol (10.5%) and β-bisabolene (7.7%) were chiefly present. Hence, the involvement of genetic factors, temperature and drought in the chemical polymorphism of P. lentiscus associated with elevation is discussed in this report.
选择了三个不同的海拔高度，研究了雌性黄连木叶片萜类化合物组成的变异性，以研究海拔梯度的变化。GC-MS 分析表明，萜类化合物的含量随海拔高度而变化。鉴定出 49 种化合物，低海拔和中海拔地区的种间变异性较高，当数据以干重为基础表示时，这些地区也表现出相同的主要成分。然而，双因素方差分析（Two-Way-ANOVA）加 Tukey 事后检验显示，单萜烃随着海拔的升高而增加，分别为低海拔、中海拔和高海拔地区的 21.7、37.5 和 221.5µg g⁻¹ dw。另一方面，应用 PCA 分析，用挥发性提取物色谱图的百分比表示数据，确定了与海拔水平相关的三种化学型。在高海拔地区（第 I 组），主要化合物为 β-石竹烯（12%）、δ-杜松烯（9.3%）和 α-蒎烯（6.3%），而在中海拔地区（第 II 组），β-石竹烯（11.5%）、δ-杜松烯（8.6%）和石竹烯氧化物（6.8%）为主要成分。在低海拔地区（第 III 组），δ-杜松烯（10.9%）、古巴醇（10.5%）和 β-双环烯（7.7%）主要存在。因此，本文讨论了遗传因素、温度和干旱在与海拔相关的黄连木化学多态性中的作用。
