Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Princess Alexandra Hospital, The University of Queensland, Woolloongabba, Brisbane, Australia.
Arch Virol. 2011 Jul;156(7):1161-71. doi: 10.1007/s00705-011-0974-3. Epub 2011 Mar 26.
Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently known miRNAs. Predicted HPV miRNAs related to miR-466, -467 and -669 were common and specific to the mucosal HPV types. Northern blot hybridization confirmed a predicted miRNA in HPV-positive cervical cancer cell lines encoded by mucosal HPVs. HPV-38 was predicted to express an miRNA conserved to human let-7a and the expression of let-7a, in HPV-38-positive non-melanoma skin cancer (NMSC) biopsies was 10-fold higher than those with HPV-positive (for other types except HPV-38) and HPV-negative NMSCs, suggesting that let-7a expression might be related to HPV-38 infection. Potential gene targets of the predicted miRNA that may aid HPV in infection and pathogenesis were also analysed.
选择了八种人乳头瘤病毒 (HPV) 类型，包括四种皮肤 HPV 类型（HPV-5、HPV-8、HPV-20 和 HPV-38）和四种黏膜 HPV 类型（HPV-6、HPV-11、HPV-16 和 HPV-18）进行这项 miRNA 研究。使用计算机程序预测前体 miRNA，并将保守的成熟 miRNA 与当前已知的 miRNA 进行比较。预测与 miR-466、-467 和 -669 相关的 HPV miRNAs 是黏膜 HPV 类型所共有的和特异的。Northern blot 杂交证实了黏膜 HPV 编码的 HPV 阳性宫颈癌细胞系中的一种预测 miRNA。HPV-38 预测表达一种与人类 let-7a 保守的 miRNA，HPV-38 阳性非黑色素瘤皮肤癌 (NMSC) 活检中 let-7a 的表达比 HPV 阳性（除 HPV-38 以外的其他类型）和 HPV 阴性 NMSC 高 10 倍，提示 let-7a 表达可能与 HPV-38 感染有关。还分析了预测 miRNA 的潜在基因靶标，这些靶标可能有助于 HPV 感染和发病机制。
