用可溶性病毒蛋白和膜蛋白诱导病毒特异性细胞毒性T淋巴细胞。
The induction of virus-specific cytotoxic T lymphocytes with solubilized viral and membrane proteins.
作者信息
Finberg R, Mescher M, Burakoff S J
出版信息
J Exp Med. 1978 Dec 1;148(6):1620-7. doi: 10.1084/jem.148.6.1620.
Abstract
Reconstituted membranes were prepared from detergent solubilized P815 (H-2d) tumor cell membranes and solubilized Sendai virus protein. These reconstituted membranes stimulated a virus-specific H-2-restricted secondary CTL response. Stimulating activity was dependent upon the presence of both viral and P815 protein in the same membrane and was restricted to the H-2 specificity present in the reconstituted membrane. Liposomes prepared from solubilized Sendai virus proteins and partially purified H-2 alloantigen also had activity for CTL induction. The results demonstrate the feasibility of using detergent solubilized membrane proteins to study antigen recognition by virus-specific, H-2 restricted cytolytic T lymphocytes.
摘要
重组膜由去污剂溶解的P815（H-2d）肿瘤细胞膜和溶解的仙台病毒蛋白制备而成。这些重组膜刺激了病毒特异性的、H-2限制性的二次CTL反应。刺激活性取决于同一膜中病毒蛋白和P815蛋白的同时存在，并且局限于重组膜中存在的H-2特异性。由溶解的仙台病毒蛋白和部分纯化的H-2同种抗原制备的脂质体也具有诱导CTL的活性。结果证明了使用去污剂溶解的膜蛋白来研究病毒特异性、H-2限制性细胞毒性T淋巴细胞对抗原识别的可行性。
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