Kodach V M, Faber D J, van Marle J, van Leeuwen T G, Kalkman J
Biomedical Engineering & Physics, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.
Opt Express. 2011 Mar 28;19(7):6131-40. doi: 10.1364/OE.19.006131.
In this work we demonstrate measurements with optical coherence tomography (OCT) of the scattering phase function in the backward direction and the scattering anisotropy parameter g. Measurements of the OCT attenuation coefficient and the backscattering amplitude are performed on calibrated polystyrene microspheres with a time-domain OCT system. From these measurements the phase function in the backward direction is determined. The measurements are described by the single scattering model and match Mie calculations very well. Measurements on Intralipid demonstrate the ability to determine the g of polydisperse samples and, for Intralipid, g = 0.35 ± 0.03 is measured, which is well in agreement with g from literature. These measurements are validated using the Intralipid particle size distribution determined from TEM measurements. Measurements of g and the scattering phase function in the backward direction can be used to monitor changes in backscattering, which can indicate morphological changes of the sample or act as contrast enhancement mechanism.
在本工作中，我们展示了利用光学相干断层扫描（OCT）测量后向散射相函数和散射各向异性参数g。使用时域OCT系统对校准后的聚苯乙烯微球进行OCT衰减系数和后向散射幅度的测量。通过这些测量确定后向散射相函数。这些测量由单次散射模型描述，并且与米氏计算结果非常吻合。对英脱利匹特（Intralipid）的测量证明了确定多分散样品g值的能力，对于英脱利匹特，测得g = 0.35 ± 0.03，这与文献中的g值非常一致。这些测量通过使用由透射电子显微镜（TEM）测量确定的英脱利匹特粒径分布进行了验证。后向散射方向上g值和散射相函数的测量可用于监测后向散射的变化，这可以指示样品的形态变化或作为对比度增强机制。
