Wen Jian, Jiang Shulian, Chen Baoan
The Affiliated Nanjing Second Hospital, Medicine School of Southeast University, Nanjing, China.
Bioelectromagnetics. 2011 May;32(4):322-4. doi: 10.1002/bem.20646. Epub 2011 Feb 22.
Our previous cellular experiments demonstrated that 100 Hz magnetic field (MF) was effective at enhancing apoptosis of liver cancer cells BEL-7402 induced by X-ray irradiation. This study was performed to further explore the possible synergism between 100 Hz MF and X-ray in treatment of hepatoma-implanted Balb/c mice. 100 Hz MF exposure with a mean flux density of 0.7 mT was performed inside an energized solenoid coil. Six MV X-ray irradiation was generated using a linear accelerator. Tumor growth and survival of mice implanted with H22 cells were evaluated by measuring the tumor diameters and overall days of survival. Six groups treated with 100 Hz MF or X-ray alone or a combination of MF and X-ray were examined. Furthermore, the effects of different numbers of MF exposure periods on tumor growth and mice survival were examined when combined with 4 Gy X-ray. Data referring to overall survival days and tumor diameters of the above groups were compared using log-rank test and Student's t-test. Our results showed that five periods of combined 100 Hz MFs and 4 Gy X-ray could significantly extend the overall days of survival and reduce the tumor size compared to MF or X-ray alone. Also, a greater number of 100 Hz MF exposure periods could further improve the survival and inhibit tumor growth in hepatoma-implanted mice when combined with 4 Gy X-ray. In conclusion, these findings suggested that 100 Hz MF could possibly synergize with 4 Gy X-ray in terms of survival improvement and tumor inhibition in hepatoma-implanted mice.
我们之前的细胞实验表明，100Hz磁场（MF）可有效增强X射线照射诱导的肝癌细胞BEL-7402的凋亡。本研究旨在进一步探讨100Hz MF与X射线在治疗肝癌移植Balb/c小鼠中的可能协同作用。在通电的螺线管线圈内进行平均通量密度为0.7mT的100Hz MF暴露。使用直线加速器产生6MV X射线照射。通过测量肿瘤直径和总体存活天数来评估接种H22细胞的小鼠的肿瘤生长和存活情况。检测了单独用100Hz MF或X射线或MF与X射线联合处理的六组。此外，当与4Gy X射线联合使用时，研究了不同次数的MF暴露期对肿瘤生长和小鼠存活的影响。使用对数秩检验和学生t检验比较上述各组的总体存活天数和肿瘤直径数据。我们的结果表明，与单独使用MF或X射线相比，100Hz MF与4Gy X射线联合处理五个周期可显著延长总体存活天数并减小肿瘤大小。此外，当与4Gy X射线联合使用时，更多次数的100Hz MF暴露期可进一步提高肝癌移植小鼠的存活率并抑制肿瘤生长。总之，这些发现表明100Hz MF在提高肝癌移植小鼠的存活率和抑制肿瘤方面可能与4Gy X射线具有协同作用。
