Joskova M, Franova S, Sadlonova V
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
Bratisl Lek Listy. 2011;112(1):9-12.
The aim of our study was to investigate the acute effect of quercetin on experimental allergic asthma after single-dose oral administration.
Airway hyperresponsiveness is one of the main features of allergic asthma. None of quercetin experimental studies analysed the acute effect of this flavonol on the reactivity of airways both, in vivo and in vitro conditions.
Our experiment was realized 21 days after the sensitization of guinea pigs with ovalbumin suspension. Changes in the reactivity of airways were studied using the whole body plethysmography in order to compare changes of the specific airway conductance between groups with and without quercetin treatment. Also changes in the reactivity of the tracheal smooth muscle dipped into the organ bath with Krebs-Henseleit solution were measured as the reaction on cumulative doses of the bronchoconstrictor mediators histamine and acetylcholine. Quercetin was added into the solution 30 minutes before the chemical mediators. The amplitude of tracheal smooth muscle precontracted with histamine or acetylcholine was used as a tracheal smooth muscle reactivity parameter in vitro.
Our results showed that quercetin (20 mg/kg) caused significant bronchodilation, both in vivo and in vitro.
Quercetin proved in laboratory conditions its ability to reduce hyperreactivity of airways as one of the main attribute of allergic asthma (Fig. 2, Ref. 23).
我们研究的目的是调查单次口服槲皮素对实验性变应性哮喘的急性影响。
气道高反应性是变应性哮喘的主要特征之一。尚无槲皮素的实验研究分析这种黄酮醇在体内和体外条件下对气道反应性的急性影响。
我们的实验在豚鼠用卵清蛋白悬液致敏21天后进行。使用全身体积描记法研究气道反应性的变化，以比较槲皮素治疗组和未治疗组之间特定气道传导率的变化。还测量了浸入含有克雷布斯-亨泽莱特溶液的器官浴中的气管平滑肌对支气管收缩介质组胺和乙酰胆碱累积剂量的反应性变化。在加入化学介质前30分钟将槲皮素加入溶液中。用组胺或乙酰胆碱预收缩的气管平滑肌振幅作为体外气管平滑肌反应性参数。
我们的结果表明，槲皮素（20毫克/千克）在体内和体外均引起显著的支气管扩张。
在实验室条件下，槲皮素证明了其作为变应性哮喘主要特征之一降低气道高反应性的能力（图2，参考文献23）。
