Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin, Ireland.
Nanomedicine. 2011 Dec;7(6):818-26. doi: 10.1016/j.nano.2011.03.005. Epub 2011 Mar 29.
Central to understanding how nanoscale objects interact with living matter is the need for reproducible and verifiable data that can be interpreted with confidence. Likely this will be the basis of durable advances in nanomedicine and nanomedical safety. To develop these fields, there is also considerable interest in advancing the first generation of theoretical models of nanoparticle (NP) uptake into cells, and NP biodistribution in general. Here we present an uptake study comparing the outcomes for free molecular dye and NPs labeled with the same dye. A simple flux-based approach is presented to model NP uptake. We find that the intracellular NP concentration grows linearly in time, and that the uptake is essentially irreversible, with the particles accumulating in lysosomes. A wide range of practical challenges, from labile dye release to NP aggregation and the need to account for cell division, are addressed to ensure that these studies yield meaningful kinetic information.
The authors present an uptake study comparing the outcomes for free molecular dye and NPs labeled with the same dye. A wide range of practical challenges are addressed including labile dye release, NP aggregation and the need to account for cell division with the goal that these studies yield meaningful kinetic information.
理解纳米级物体与生命物质相互作用的核心是需要可重复和可验证的数据，以便有信心进行解释。这可能是纳米医学和纳米医学安全方面持久进展的基础。为了发展这些领域，人们也非常有兴趣推进第一代纳米颗粒（NP）进入细胞摄取和 NP 生物分布的理论模型。在这里，我们进行了一项摄取研究，比较了带有相同染料标记的自由分子染料和 NPs 的结果。提出了一种基于通量的方法来模拟 NP 的摄取。我们发现细胞内 NP 浓度随时间呈线性增长，摄取基本上是不可逆的，颗粒在溶酶体中积累。解决了一系列实际挑战，包括不稳定染料的释放、NP 聚集以及需要考虑细胞分裂，以确保这些研究产生有意义的动力学信息。
作者进行了一项摄取研究，比较了带有相同染料标记的自由分子染料和 NPs 的结果。解决了一系列实际挑战，包括不稳定染料的释放、NP 聚集以及需要考虑细胞分裂，目的是使这些研究产生有意义的动力学信息。
