Redolfi-Bristol Davide, Yamamoto Kenta, Zhu Wenliang, Mazda Osam, Riello Pietro, Marin Elia, Pezzotti Giuseppe
Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, Kyoto 606-8585, Japan.
Dipartimento di Scienze Molecolari e Nanosistemi, Università Ca' Foscari di Venezia, Via Torino 155, 30172 Venezia, Italia.
ACS Appl Mater Interfaces. 2025 Mar 26;17(12):18124-18133. doi: 10.1021/acsami.5c00380. Epub 2025 Mar 18.
Selenium nanoparticles (SeNPs) exhibit significant potential in biomedical applications due to their antimicrobial, anticancer, and anti-inflammatory properties. In this study, we synthesized biocompatible SeNPs and employed confocal Raman microspectroscopy to map their distribution within human dermal fibroblast (HDF) cells. SeNPs possess a distinctive Raman band placed outside the cellular fingerprint region, which facilitates its detection and precise Raman imaging. Viability assays revealed that SeNPs exhibit cytotoxic effects only at the highest concentrations and for long exposure times while resulting in no harmful effects during all of the other treatments. For the first time, we achieved three-dimensional (3D) Raman mapping of SeNPs within cells, providing insights into their cellular penetration. Additionally, two-dimensional (2D) Raman mapping performed at different times and at sublethal concentrations demonstrated dynamic uptake and confirmed internalization. These findings highlight the effectiveness of SeNPs for biomedical imaging and therapeutic applications, offering an additional approach to studying nanoparticle-cell interactions.
硒纳米颗粒（SeNPs）因其抗菌、抗癌和抗炎特性而在生物医学应用中展现出巨大潜力。在本研究中，我们合成了具有生物相容性的硒纳米颗粒，并利用共聚焦拉曼光谱对其在人皮肤成纤维细胞（HDF）内的分布进行成像。硒纳米颗粒具有一个位于细胞指纹区域之外的独特拉曼峰，这有助于其检测和精确的拉曼成像。活力测定表明，硒纳米颗粒仅在最高浓度和长时间暴露时才表现出细胞毒性作用，而在所有其他处理过程中均无有害影响。我们首次实现了细胞内硒纳米颗粒的三维（3D）拉曼成像，深入了解了它们的细胞穿透情况。此外，在不同时间和亚致死浓度下进行的二维（2D）拉曼成像显示了动态摄取并证实了内化过程。这些发现突出了硒纳米颗粒在生物医学成像和治疗应用中的有效性，为研究纳米颗粒与细胞的相互作用提供了另一种方法。
