Li Chen, Zhao Yifang, Zhang Wenfeng, Zhang Wei
State Key Laboratory Breeding Base of Basic Science of Stomatology, Ministry of Education, Wuhan University, Wuhan, China.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Jul;112(1):81-9. doi: 10.1016/j.tripleo.2010.11.032. Epub 2011 Mar 31.
This study was performed to determine whether T(H)17 cells are involved in the development and metastasis of head and neck squamous cell carcinomas (HNSCCs).
T(H)17 cells frequencies in 67 HNSCC patients and 21 healthy volunteers were examined by flow cytometric analysis. T(H)17 cell-related cytokines in serum (interleukin (IL) 17, transforming growth factor (TGF) β, and IL-6) were evaluated by using enzyme-linked immunosorbent assay.
It was discovered that the higher frequency of T(H)17 cells was in HNSCCs patients (1.0 ± 0.4%). The cell proportions and related cytokine concentrations were consistent with the tumor TNM stage. The IL-6 concentration showed positive correlation with the frequency of T(H)17 cells (r = 0.661) and IL-17 levels (r = 0.597). The TGF-β concentration showed a positive correlation with IL-17 (r = 0.626) but no relationship with T(H)17 cells (r = 0.431).
The present data suggested that T(H)17 cells may be involved in tumor growth and metastasis of HNSCCs. IL-6 may play an important role in T(H)17 cell differentiation and functions, and TGF-β may be related to IL-17 secretion but not to the differentiation of T(H)17 cells.
本研究旨在确定辅助性T细胞17（Th17）是否参与头颈部鳞状细胞癌（HNSCC）的发生发展及转移。
采用流式细胞术分析检测67例HNSCC患者和21名健康志愿者体内Th17细胞的频率。采用酶联免疫吸附测定法评估血清中Th17细胞相关细胞因子（白细胞介素（IL）-17、转化生长因子（TGF）-β和IL-6）。
发现HNSCC患者体内Th17细胞频率较高（1.0±0.4%）。细胞比例及相关细胞因子浓度与肿瘤TNM分期一致。IL-6浓度与Th17细胞频率（r = 0.661）和IL-17水平（r = 0.597）呈正相关。TGF-β浓度与IL-17呈正相关（r = 0.626），但与Th17细胞无相关性（r = 0.431）。
目前的数据表明，Th17细胞可能参与HNSCC的肿瘤生长和转移。IL-6可能在Th17细胞分化和功能中起重要作用，而TGF-β可能与IL-17分泌有关，但与Th17细胞分化无关。
