College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Appl Environ Microbiol. 2011 Jun;77(11):3884-7. doi: 10.1128/AEM.00190-11. Epub 2011 Apr 1.
DNA-based stable-isotope probing was applied to identify the active microorganisms involved in syntrophic butyrate oxidation in paddy field soil. After 14 and 21 days of incubation with [U-(13)C]butyrate, the bacterial Syntrophomonadaceae and the archaeal Methanosarcinaceae and Methanocellales incorporated substantial amounts of (13)C label into their nucleic acids. Unexpectedly, members of the Planctomycetes and Chloroflexi were also labeled with (13)C by yet-unclear mechanisms.
采用基于 DNA 的稳定同位素探测法来鉴定稻田土壤中参与丁酸共氧化的活性微生物。在与 [U-(13)C]丁酸共培养 14 和 21 天后,细菌的互营单胞菌科和古菌的产甲烷菌科和甲烷杆菌目大量将 (13)C 标记掺入到它们的核酸中。出乎意料的是,通过尚不清楚的机制,浮霉菌门和绿弯菌门的成员也被 (13)C 标记。