定量 PCR 靶向 Desulfovibrio 16S rRNA 基因对人粪便中硫酸盐还原菌丰度的高估。
Overestimation of the abundance of sulfate-reducing bacteria in human feces by quantitative PCR targeting the Desulfovibrio 16S rRNA gene.
机构信息
CSIRO Food and Nutritional Sciences, Gate 13, Kintore Avenue, Adelaide 5000, South Australia, Australia.
出版信息
Appl Environ Microbiol. 2011 May;77(10):3544-6. doi: 10.1128/AEM.02851-10. Epub 2011 Apr 1.
Abstract
The dominant genus of sulfate-reducing bacteria (SRB) in humans is Desulfovibrio, and quantitative PCR (QPCR) targeting the 16S rRNA gene is often used in assays. We show that the 16S rRNA gene assay overestimated SRB abundance in feces from 24 adults compared to QPCR assays using primers targeting two genes involved in SRB energy metabolism.
摘要
在人类中，硫酸盐还原菌（SRB）的优势属是脱硫弧菌，针对 16S rRNA 基因的定量 PCR（QPCR）常用于检测。我们发现，与针对参与 SRB 能量代谢的两个基因的引物的 QPCR 检测相比，16S rRNA 基因检测高估了 24 名成年人粪便中 SRB 的丰度。
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