Departamento de Microbiología y Parasitología, Centro de Investigaciones Biológicas (CIBUS), Universidad de Santiago de Compostela, Campus Sur 15782, Santiago de Compostela, Spain.
Skretting, Ctra. de la Estación, Cojobar, Burgos 09620, Spain.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):425-429. doi: 10.1099/ijs.0.025122-0. Epub 2011 Apr 1.
A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09(T), was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum 'Bacteroidetes'). Sequence similarities between the isolate and type strains of other members of the genus were 93.1-97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C(15 : 0) (24.8 %), iso-C(15 : 0) 3-OH (18.0 %), anteiso-C(15 : 0) (8.1 %), C(15 : 1)ω6c (6.9 %) and iso-C(15 : 1) (6.2 %). Genotypic and phenotypic data indicate that strain 35/09(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09(T) ( = CECT 7612(T) = NCIMB 14598(T)).
一株新型革兰氏阴性滑行杆状细菌，菌株 35/09(T)，从患病的欧洲鲈鱼（Dicentrarchus labrax L.）中分离得到，分离自西班牙。菌落呈浅黄色，边缘不均匀，不附着在琼脂上。该菌株的 DNA G+C 含量为 31.3 mol%。16S rRNA 基因序列分析表明，该菌株与 Tenacibaculum 属（Flavobacteriaceae 科，'Bacteroidetes' 门）有关。该菌株与属内其他模式菌株的 16S rRNA 基因序列相似性为 93.1-97.3%。主要脂肪酸（占总脂肪酸的>5%）为 iso-C(15:0)（24.8%）、iso-C(15:0) 3-OH（18.0%）、anteiso-C(15:0)（8.1%）、C(15:1)ω6c（6.9%）和 iso-C(15:1)（6.2%）。基因型和表型数据表明，菌株 35/09(T)应归类为 Tenacibaculum 属的一个新型种的代表，建议将其命名为 Tenacibaculum dicentrarchi sp. nov.；其模式菌株为 35/09(T)（=CECT 7612(T)=NCIMB 14598(T)）。
