Ahmadu A A, Onanuga A, Aquino R
Department of Pharmaceutical and Medicinal Chemistry, Niger-Delta University,Wilberforce Jsland, Bayelsa-Nigeria.
Afr J Tradit Complement Altern Med. 2010 Apr 3;7(3):225-30. doi: 10.4314/ajtcam.v7i3.54780.
The bioactive N-butanol fraction of the ethanol extract of the leaves of Cissus ibuensis was fractionated over silica Gel column to give Quercetin 3-O-rutinoside (I) and mixtures of Flavonoids (A2). A2 was fractionated using reverse phase HPLC to give Kaempferol 3-O-α-rhamnopyranosyl (1→6)-β-D-galactopyranoside (II), Kaempferol 3-O-rutinoside(III) and Kaempferol3-O-α-rhamnopyranosyl (1→6)-α-rhamnopyranosyl(1→2)-β-D-galactopyranoside (IV). The structures were elucidated by NMR spectroscopy and compared with literature.
对滇南崖爬藤叶片乙醇提取物的生物活性正丁醇部分进行硅胶柱层析，得到槲皮素3 - O - 芸香糖苷（I）和黄酮类混合物（A2）。使用反相高效液相色谱对A2进行分离，得到山柰酚3 - O - α - 鼠李吡喃糖基（1→6）- β - D - 吡喃半乳糖苷（II）、山柰酚3 - O - 芸香糖苷（III）和山柰酚3 - O - α - 鼠李吡喃糖基（1→6）- α - 鼠李吡喃糖基（1→2）- β - D - 吡喃半乳糖苷（IV）。通过核磁共振光谱对结构进行解析，并与文献进行比较。
