Applied Research Department, Philip Morris International, Quai Jeanrenaud 5, 2000, Neuchatel, Switzerland.
Theor Appl Genet. 2011 Jul;123(2):219-30. doi: 10.1007/s00122-011-1578-8. Epub 2011 Apr 2.
Tobacco (Nicotiana tabacum L.) is a species in the large family of the Solanaceae and is important as an agronomic crop and as a model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available that can be used for genome analysis, genetic mapping and breeding. We report here on the development and characterization of 5,119 new and functional microsatellite markers and on the generation of a high-resolution genetic map for the tetraploid tobacco genome. The genetic map was generated using an F2 mapping population derived from the intervarietal cross of Hicks Broadleaf × Red Russian and merges the polymorphic markers from this new set with those from a smaller set previously used to produce a lower density map. The genetic map described here contains 2,317 microsatellite markers and 2,363 loci, resulting in an average distance between mapped microsatellite markers which is less than 2 million base pairs or 1.5 cM. With this new and expanded marker resource, a sufficient number of markers are now available for multiple applications ranging from tobacco breeding to comparative genome analysis. The genetic map of tobacco is now comparable in marker density and resolution with the best characterized genomes of the Solanaceae: tomato and potato.
烟草（Nicotiana tabacum L.）是茄科大家族中的一个物种，作为一种农业作物和植物生物技术的模式系统非常重要。尽管它很重要，但可用于基因组分析、遗传图谱构建和育种的分子标记资源有限。我们在此报告了 5119 个新的和功能的微卫星标记的开发和特征，并报告了四倍体烟草基因组的高分辨率遗传图谱。该遗传图谱是使用 Hicks 宽叶×Red Russian 品种间杂交的 F2 作图群体生成的，它将来自这个新集合的多态性标记与以前用于生成较低密度图谱的较小集合的标记合并在一起。本文所描述的遗传图谱包含 2317 个微卫星标记和 2363 个位点，导致映射的微卫星标记之间的平均距离小于 200 万个碱基或 1.5cM。有了这个新的和扩展的标记资源，现在有足够数量的标记可用于从烟草育种到比较基因组分析的多种应用。烟草的遗传图谱在标记密度和分辨率方面现在可与茄科中特征最明显的基因组（番茄和土豆）相媲美。
