Saygili Ibrahim, Kandemir Nejdet, Kinay Ahmet, Aytac Selim, Ayan Ali Kemal
Department of Field Crops, Faculty of Agriculture, Tokat Gaziosmanpasa University, Tokat, Turkey.
Department of Biology, Faculty of Sciences and Letters, Ankara Hacı Bayram Veli University, Polatlı, Ankara, Turkey.
Mol Biol Rep. 2022 Dec;49(12):11351-11358. doi: 10.1007/s11033-022-07815-1. Epub 2022 Aug 1.
Turkey is one of the traditional oriental tobacco producing countries. Oriental tobaccos produced in Turkey are ecotypes specific to certain regions, local varieties and landraces, and they have unique characteristics. The present study was conducted to reveal the general and interregional genetic diversity levels of tobacco genotypes collected from different regions of Turkey based on DNA markers.
A total of 319 ecotypes/lines of Turkish Oriental Tobaccos collected from different regions (Aegean Region, Marmara Region, Karadeniz Region, and East and Southeast Anatolia Regions) of Turkey and cultivars Xanthi 81, Xanthi 2A, Canik Sitmasuyu, Katerini, Canik 190-5 and NC 55 were subjected to SSR marker analysis. Eighty-nine alleles were obtained from 21 markers examined, and the average number of alleles per marker was 4.05. A total of 314 unique tobacco genotypes were found among 319 plants evaluated using 19 polymorphic SSR markers. In the dendrogram constructed using SSR marker data, genotypes were divided into six clades based on geographic regions and levels of genetic diversity.
The presence of 314 unique genotypes among 319 tobacco plants evaluated indicated the high level of genetic diversity among Turkish tobaccos. While some of them were N. rustica tobaccos, some others were reminiscent of semi-oriental tobaccos. These genotypes merit further agronomic and technological characterizations, which could allow their utilization in future tobacco breeding.
土耳其是传统的东方烟草生产国之一。土耳其生产的东方烟草是特定地区的生态型、地方品种和农家品种，具有独特的特征。本研究旨在基于DNA标记揭示从土耳其不同地区收集的烟草基因型的总体和区域间遗传多样性水平。
对从土耳其不同地区（爱琴海地区、马尔马拉地区、黑海地区以及东安纳托利亚和东南安纳托利亚地区）收集的319个土耳其东方烟草生态型/品系以及品种Xanthi 81、Xanthi 2A、Canik Sitmasuyu、Katerini、Canik 190 - 5和NC 55进行SSR标记分析。从检测的21个标记中获得了89个等位基因，每个标记的平均等位基因数为4.05。使用19个多态性SSR标记在评估的319株植物中总共发现了314个独特的烟草基因型。在使用SSR标记数据构建的聚类图中，基因型根据地理区域和遗传多样性水平分为六个分支。
在评估的319株烟草植物中存在314个独特的基因型，这表明土耳其烟草具有高度的遗传多样性。其中一些是黄花烟草，另一些则让人联想到半东方烟草。这些基因型值得进一步进行农艺和技术特性鉴定，以便在未来的烟草育种中加以利用。
