Liu Yutong, Yuan Guangdi, Si Huan, Sun Ying, Jiang Zipeng, Liu Dan, Jiang Caihong, Pan Xuhao, Yang Jun, Luo Zhaopeng, Zhang Jianfeng, Ren Min, Pan Yi, Sun Kefan, Meng He, Wen Liuying, Xiao Zhiliang, Feng Quanfu, Yang Aiguo, Cheng Lirui
Key Laboratory of Tobacco Improvement and Biotechnology, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Zhengzhou Tobacco Research Institute, China Tobacco Gene Research Centre, Zhengzhou, China.
Front Plant Sci. 2022 Jun 6;13:878267. doi: 10.3389/fpls.2022.878267. eCollection 2022.
Agronomic traits such as plant height (PH), leaf number (LN), leaf length (LL), and leaf width (LW), which are closely related to yield and quality, are important in tobacco ( L.). To identify quantitative trait loci (QTLs) associated with agronomic traits in tobacco, 209 recombinant inbred lines (RILs) and 537 multiparent advanced generation intercross (MAGIC) lines were developed. The biparental RIL and MAGIC lines were genotyped using a 430 K single-nucleotide polymorphism (SNP) chip assay, and their agronomic traits were repeatedly evaluated under different conditions. A total of 43 QTLs associated with agronomic traits were identified through a combination of linkage mapping (LM) and association mapping (AM) methods. Among these 43 QTLs, three major QTLs, namely , and , were repeatedly identified by the use of various genetically diverse populations across different environments. The candidate genes for these major QTLs were subsequently predicted. Validation and utilization of the major QTL for the improvement of LW in tobacco were investigated. These results could be applied to molecular marker-assisted selection (MAS) for breeding important agronomic traits in tobacco.
株高（PH）、叶片数（LN）、叶长（LL）和叶宽（LW）等农艺性状与烟草产量和品质密切相关，对烟草十分重要。为了鉴定烟草中与农艺性状相关的数量性状基因座（QTL），构建了209个重组自交系（RIL）和537个多亲本高世代杂交（MAGIC）群体。利用430K单核苷酸多态性（SNP）芯片分析法对双亲本RIL群体和MAGIC群体进行基因分型，并在不同条件下对其农艺性状进行多次评估。通过连锁图谱（LM）和关联图谱（AM）相结合的方法，共鉴定出43个与农艺性状相关的QTL。在这43个QTL中，通过在不同环境下使用各种遗传多样的群体，反复鉴定出了三个主要QTL，即 、 和 。随后预测了这些主要QTL的候选基因。研究了主要QTL 对改良烟草叶宽的验证和利用。这些结果可应用于烟草重要农艺性状育种的分子标记辅助选择（MAS）。
