Wang Fu-Rong, Yang Xiu-Wei, Zhang Ying, Liu Jian-Xun, Yang Xin-Bao, Liu Yang, Shi Ren-Bing
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, China.
J Asian Nat Prod Res. 2011 Apr;13(4):319-29. doi: 10.1080/10286020.2011.556090.
Three new isoflavone glycosides, 3'-methoxydaidzein-7,4'-di-O-β-d-glucopyranoside (1), biochanin A-8-C-β-d-apiofuranosyl-(1 → 6)-O-β-d-glucopyranoside (2), daidzein-7-O-β-d-glucopyranosyl-(1 → 4)-O-β-d-glucopyranoside (3), and a new natural isoflavone glycoside, daidzein-7-O-α-d-glucopyranosyl-(1 → 4)-O-β-d-glucopyranoside (4) were isolated along with 18 known isoflavones from the EtOAc and n-BuOH fractions of the aqueous extraction of Tongmai granules. All the isoflavones were obtained and determined for the first time from Tongmai granules. The structures of these compounds were elucidated by spectral methods. It was confirmed that the compounds 1-4 were originally from Puerariae Lobatae Radix based on HPLC-DAD analysis of the crude drug extract. The isoflavones isolated were tested for their antioxidative activities by measuring the capacities of scavenging the 2,2'-diphenyl-1-picrylhydrazyl radical.
从通脉颗粒水提取物的乙酸乙酯和正丁醇部位分离得到3个新的异黄酮苷，即3'-甲氧基大豆苷元-7,4'-二-O-β-D-吡喃葡萄糖苷（1）、鹰嘴豆芽素A-8-C-β-D-芹菜呋喃糖基-(1→6)-O-β-D-吡喃葡萄糖苷（2）、大豆苷元-7-O-β-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃葡萄糖苷（3），以及1个新的天然异黄酮苷，大豆苷元-7-O-α-D-吡喃葡萄糖基-(1→4)-O-β-D-吡喃葡萄糖苷（4），同时还分离得到18个已知异黄酮。所有异黄酮均首次从通脉颗粒中获得并鉴定。通过光谱方法阐明了这些化合物的结构。基于对生药提取物的高效液相色谱-二极管阵列检测分析，证实化合物1-4最初来源于葛根。对分离得到的异黄酮进行了抗氧化活性测试，通过测定清除2,2'-二苯基-1-苦基肼自由基的能力来评估。
