Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Virus Res. 2011 Jun;158(1-2):289-93. doi: 10.1016/j.virusres.2011.03.028. Epub 2011 Apr 4.
Using swine anal swab or liver as inocula, cell-culture systems were developed for swine hepatitis E virus (HEV) in swine cells (IBRS-2) and human cells (A549). Both positive and negative strand of swine HEV RNA were detected continuously. Cytopathic effect appeared from passage 8 in IBRS-2 and passage 22 in A549. Viral antigen was detected by indirect immunofluorescent assay in infected cells. Progenies harbored mutations in the third nucleotide of codon. Amino acid changes occurred in passage 8 in IBRS-2 and rescued in passage 10. Full-length genome sequence of a swine HEV isolate from liver was determined to be genotype 4. Taken together, our data suggest that swine HEV is able to replicate in both swine and human cells in vitro.
使用猪肛门拭子或肝脏作为接种物，在猪细胞（IBRS-2）和人细胞（A549）中建立了猪戊型肝炎病毒（HEV）的细胞培养系统。连续检测到猪 HEV RNA 的正链和负链。在 IBRS-2 中从第 8 代和 A549 中从第 22 代开始出现细胞病变效应。通过间接免疫荧光法在感染细胞中检测到病毒抗原。在后代中发现密码子第三核苷酸发生突变。在 IBRS-2 中第 8 代发生氨基酸变化，并在第 10 代得到挽救。从肝脏分离的一株猪 HEV 分离株的全长基因组序列确定为基因型 4。综上所述，我们的数据表明猪 HEV 能够在体外的猪和人细胞中复制。
