中国山东省4型戊型肝炎病毒的血清流行率及进化动态
Seroprevalence and evolutionary dynamics of genotype 4 hepatitis E virus in Shandong Province, China.
作者信息
Yang Dong, Jiang Mei, Jin Min, Qiu Zhi-Gang, Shen Zhi-Qiang, Cui Wei-Hong, Wang Da-Ning, Gong Lian-Feng, Li Bo, Wang Xin-Wei, Li Jun-Wen
机构信息
Dong Yang, Min Jin, Zhi-Gang Qiu, Zhi-Qiang Shen, Da-Ning Wang, Xin-Wei Wang, Jun-Wen Li, Department of Environment and Health, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
出版信息
World J Gastroenterol. 2014 Jun 28;20(24):7955-63. doi: 10.3748/wjg.v20.i24.7955.
AIM
To investigate the seroprevalence and evolutionary dynamics of hepatitis E virus (HEV) and assess the ancestor of HEVs in China's Shandong Province.
METHODS
A total of 2028 serum, 60 fecal and 82 bile samples were collected from the general human population, patients and swine, respectively. This seroepidemiological study was conducted using an immunnosorbent assay and HEV RNA was detected by the reverse transcription-nested polymerase chain reaction (RT-nPCR) method. Complete genome sequences of the prevalent strains (CH-YT-HEV01, CH-YT-HEV02 and CH-YT-sHEV01) were determined, and the sequences were analyzed phylogenetically. In addition, the evolutionary dynamics of three HEV isolates were determined using the framework of coalescent analysis in the program package BEAST, and the time of the most recent common ancestors (TMRCAs) of China-indigenous genotype 4 HEV isolates was calculated.
RESULTS
The overall viral burden in the general human population was 0.1%, and the positive rates of anti-HEV IgG and IgM in the serum specimens were 25.1% (509/2028) and 2.3% (51/2028), respectively. In addition, IgG positivity increased with age. The phylogenetic analysis based on the full-length nucleotide sequences showed that the strain CH-YT-HEV02 was directly related to CH-YT-sHEV01 with a 94% identity, suggesting that they were involved in cross-species transmission. The isolate CH-YT-HEV01 was close to HB-3 and CHN-SD-sHEV with a bootstrap value of 100%, sharing a 96.1%-96.4% identity with each other. Surprisingly, the HB-3 strain was a representative strain prevalent in swine in Hubei, and the isolate CHN-SD-sHEV was obtained from swine in Shandong in a previous report. TMRCA for the clade of CH-YT-HEV01 and HB-3 was 2003, which was consistent with the TMRCA for the clade of CHN-SD-sHEV and HB-3, and they were both earlier than the TMRCA for the clade of CH-YT-HEV01 and CHN-SD-sHEV (2004).
CONCLUSION
The strains CH-YT-HEV01, CHN-SD-sHEV and HB-3 are involved in trans-regional transmission, and the ancestors of HEVs in Shandong come from Hubei Province.
目的
调查戊型肝炎病毒（HEV）的血清流行率和进化动态，并评估中国山东省HEV的祖先。
方法
分别从普通人群、患者和猪中收集了2028份血清、60份粪便和82份胆汁样本。本血清流行病学研究采用免疫吸附试验进行，HEV RNA通过逆转录巢式聚合酶链反应（RT-nPCR）方法检测。测定了流行株（CH-YT-HEV01、CH-YT-HEV02和CH-YT-sHEV01）的完整基因组序列，并对序列进行了系统发育分析。此外，使用程序包BEAST中的合并分析框架确定了三株HEV分离株的进化动态，并计算了中国本土4型HEV分离株的最近共同祖先时间（TMRCA）。
结果
普通人群中的总体病毒载量为0.1%，血清标本中抗HEV IgG和IgM的阳性率分别为25.1%（509/2028）和2.3%（51/2028）。此外，IgG阳性率随年龄增加而升高。基于全长核苷酸序列的系统发育分析表明，CH-YT-HEV02株与CH-YT-sHEV01直接相关，同一性为94%，表明它们参与了跨物种传播。CH-YT-HEV01分离株与HB-3和CHN-SD-sHEV接近，自展值为100%，彼此之间的同一性为96.1%-96.4%。令人惊讶的是，HB-3株是湖北省猪中流行的代表性毒株，CHN-SD-sHEV分离株是先前报告中从山东猪中获得的。CH-YT-HEV01和HB-3分支的TMRCA为2003年，这与CHN-SD-sHEV和HB-3分支的TMRCA一致，且它们均早于CH-YT-HEV01和CHN-SD-sHEV分支的TMRCA（2004年）。
结论
CH-YT-HEV01、CHN-SD-sHEV和HB-3株参与了跨区域传播，山东省HEV的祖先来自湖北省。
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