Joo Jeong In, Yun Jong Won
Department of Biotechnology, Daegu University, Kyungsan, Korea.
Cell Physiol Biochem. 2011;27(3-4):327-40. doi: 10.1159/000327959. Epub 2011 Apr 1.
Different responses to a high fat diet (HFD) can occur even within a group of animals with the same genetic background, such as obesity-prone (OP) or obesity-resistant (OR) phenotypes, on the same feeding. To explain these phenotypes, we performed an analysis of gene expression differences in brown (BAT) and white adipose tissue (WAT) of OP and OR rats. Microarray analysis of transcripts revealed that 91 and 53 genes showed significant differences in expression between the BAT and WAT gene, respectively. Surprisingly, a majority of these genes were significantly down-regulated in adipose tissues in response to HFD feeding. K-means clustering of the expression levels of these genes identified 4 distinct groupings of genes with significant expression levels. Only a limited number of genes were significantly regulated in adipose tissues in response to HFD feeding, whereas expression levels of a large number of genes differed significantly between OP and OR rat. Our observations support that distinct discrepancies exist in gene-expression regulations in adipose tissues, and that alteration likely resulted from significant differences in genes encoding metabolic enzymes. To the best of our knowledge, this study provided the first direct comparison of gene-expression changes between OP and OR rats.
即使在同一饲养条件下，具有相同遗传背景的一组动物，如易肥胖（OP）或抗肥胖（OR）表型的动物，对高脂肪饮食（HFD）也可能出现不同反应。为了解释这些表型，我们对OP和OR大鼠的棕色脂肪组织（BAT）和白色脂肪组织（WAT）中的基因表达差异进行了分析。转录本的微阵列分析显示，BAT和WAT基因之间分别有91个和53个基因的表达存在显著差异。令人惊讶的是，这些基因中的大多数在高脂饮食喂养后在脂肪组织中显著下调。对这些基因表达水平进行K均值聚类，确定了4个具有显著表达水平的不同基因分组。在高脂饮食喂养后，脂肪组织中只有有限数量的基因受到显著调控，而OP和OR大鼠之间大量基因的表达水平存在显著差异。我们的观察结果支持脂肪组织中基因表达调控存在明显差异，并且这种改变可能是由于编码代谢酶的基因存在显著差异所致。据我们所知，本研究首次对OP和OR大鼠之间的基因表达变化进行了直接比较。
