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正交试验设计优化新疆芍药多糖提取工艺

Orthogonal test design for optimization of the extraction of polysaccharide from Paeonia sinjiangensis K.Y. Pan.

作者信息

Tian Shuge, Zhou Xiaoying, Gong Haiyan, Ma Xiuming, Zhang Fan

机构信息

Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, XinJiang, China.

出版信息

Pharmacogn Mag. 2011 Jan;7(25):4-8. doi: 10.4103/0973-1296.75874.

DOI:10.4103/0973-1296.75874
PMID:21472071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3065155/
Abstract

BACKGROUND

Paeonia sinjiangensis K.Y. Pan is a perennial herb belonging to the ranunculaceae family and it is one of the most important crude drugs in traditional Chinese medicine. In this article, Paeonia sinjiangensis K.Y. Pan rich in polysaccharide is used as an experimental material.

MATERIALS AND METHOD

Study the effects of proportion, temperature, times and time taken for the extraction yield of polysaccharide through a single-factor exploration. Then, through an orthogonal experiment (L(9)(3)(4)), it was investigated to get the best extraction conditions.

RESULTS

The results showed that the ratio of solvent to raw material, number of extractions and duration of extraction were the main variables that influenced the yields of extracts. The separation procedure of precipitation with alcohol and the purification from the removing proteins were deeply analyzed. Meanwhile the contents of polysaccharide were determined by anthrone colorimetry.

CONCLUSION

The highest yield was obtained when the ratio of solvent to raw material, number of extractions, and duration of extraction were 8:1, 2, and 1.5 h, respectively. The content of soluble polysaccharide is 51.57%.

摘要

背景

新疆芍药是毛茛科多年生草本植物，是传统中药中最重要的药材之一。本文以富含多糖的新疆芍药为实验材料。

材料与方法

通过单因素探索研究比例、温度、次数及提取时间对多糖提取率的影响。然后，通过正交试验（L(9)(3)(4)）探究最佳提取条件。

结果

结果表明，溶剂与原料的比例、提取次数和提取时间是影响提取物产量的主要变量。深入分析了醇沉分离过程和去除蛋白质的纯化过程。同时采用蒽酮比色法测定多糖含量。

结论

当溶剂与原料的比例、提取次数和提取时间分别为8:1、2次和1.5小时时，多糖产量最高。可溶性多糖含量为51.57%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/ab6011d815b7/PM-7-4-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/fe31e170714c/PM-7-4-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/68d593d5e9d6/PM-7-4-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/c3dad4eed5e7/PM-7-4-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/417642c5ac8d/PM-7-4-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/ab6011d815b7/PM-7-4-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/fe31e170714c/PM-7-4-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/68d593d5e9d6/PM-7-4-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/c3dad4eed5e7/PM-7-4-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/417642c5ac8d/PM-7-4-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b04/3065155/ab6011d815b7/PM-7-4-g005.jpg

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