大蒜鳞茎水提取物对大鼠离体气管的舒张作用。

The relaxant effect induced by Allium sativum L. bulb aqueous extract on rat isolated trachea.

作者信息

Fehri Badreddine, Ahmed Mueen K K, Aiache Jean-Marc

机构信息

Department of Pharmacology and Toxicology, Tunisia Pharmaceutical Industries Company (SIPHAT), Fondouk Choucha-Radès, 2013 Ben Arous, Tunisia.

出版信息

Pharmacogn Mag. 2011 Jan;7(25):14-8. doi: 10.4103/0973-1296.75879.

DOI:10.4103/0973-1296.75879

PMID:21472073

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3065150/

Abstract

BACKGROUND

Garlic plays an important role in complementary and alternative medicine. Most people believe in and use herbal products even when they have not been as thoroughly researched as garlic. Garlic is also known for its beneficial effects on the cardiovascular system.

MATERIALS AND METHODS

The relaxant effect of Allium sativum L. bulb aqueous extract (ASBAE) containing 0.06%-0.10% of allicin was studied on isolated smooth muscle of trachea of rats precontracted using acetylcholine (10(-5) M).

RESULTS

It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7). Pretreatments with mepyramine (10(-7) M), methysergide (10(-7) M), caffeine (10(-6) M), theophylline (10(-6) M), nifedipine (10(-6) M), and dipyridamole (10(-6) M) did not alter ASBAE concentration-response curves. In turn, concentration-response curves to ASBAE were significantly shifted toward right in the presence of aspirin (3.10(-3) M), indomethacin (10(-6) M), prazosin (10(-6) M), and propranolol (10(-7) M).

CONCLUSION

It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

摘要

背景

大蒜在补充和替代医学中发挥着重要作用。即使许多草药产品没有像大蒜那样经过充分研究，大多数人仍然相信并使用它们。大蒜对心血管系统的有益作用也广为人知。

材料与方法

研究了含有0.06%-0.10%大蒜素的大蒜鳞茎水提取物（ASBAE）对用乙酰胆碱（10⁻⁵ M）预收缩的大鼠离体气管平滑肌的舒张作用。

结果

发现ASBAE诱导剂量依赖性舒张，记录的半数有效浓度（EC₅₀）值为71.87±5.90 μg/mL（n = 7）。用美吡拉敏（10⁻⁷ M）、甲基麦角新碱（10⁻⁷ M）、咖啡因（10⁻⁶ M）、茶碱（10⁻⁶ M）、硝苯地平（10⁻⁶ M）和双嘧达莫（10⁻⁶ M）预处理不会改变ASBAE的浓度-反应曲线。相反，在阿司匹林（3×10⁻³ M）、吲哚美辛（10⁻⁶ M）、哌唑嗪（10⁻⁶ M）和普萘洛尔（10⁻⁷ M）存在的情况下，ASBAE的浓度-反应曲线显著右移。