Department of Kinesiology, Indiana University, Bloomington, IN 47405, USA.
Eur J Appl Physiol. 2012 Jan;112(1):33-42. doi: 10.1007/s00421-011-1946-8. Epub 2011 Apr 7.
The aim of this investigation was to establish whether changes in oxidative stress and endothelial function following acute aerobic exercise are dose-dependent. Ten healthy trained men completed four exercise sessions: 50% VO(2peak) for 30 min (moderate intensity moderate duration, MIMD), 50% VO(2peak) for 60 min (moderate intensity long duration, MILD), 80% VO(2peak) for 30 min (high intensity moderate duration, HIMD), and 80% VO(2peak) for the time to reach the caloric equivalent of MIMD (high intensity short duration, HISD). Thiobarbituric acid reactive substances (TBARS) were measured as an index of oxidative stress and brachial artery flow-mediated dilation (FMD) was assessed as an index of endothelial function. Variables were measured at baseline, immediately post-exercise, 1 and 2 h post-exercise. Both HIMD (14.2 ± 2.5 μmol/L) and HISD (14.7 ± 1.9 μmol/L) TBARS differed from MIMD (11.8 ± 1.5 μmol/L) immediately post-exercise. TBARS increased from pre to immediately post-exercise for HIMD (12.6 ± 2.1 vs.14.2 ± 2.5 μmol/L) and HISD (12.3 ± 2.8 vs. 14.7 ± 1.9 μmol/L). Both MIMD (7.2 ± 2.2%) and HISD (7.6 ± 2.7%) FMD immediately post-exercise were greater than HIMD (4.7 ± 2.2%). An increase of FMD from pre to immediately post-exercise was found for MIMD (5.0 ± 2.5 vs. 7.2 ± 2.2%) and HISD (5.9 ± 2.4 vs. 7.6 ± 2.7%). These data suggest that acute exercise-induced TBARS are exercise intensity-dependent whereas FMD appears to improve following energy expenditure equivalent to 30 min 50% VO(2peak), regardless of intensity or duration.
本研究旨在确定急性有氧运动后氧化应激和内皮功能的变化是否与剂量有关。10 名健康训练有素的男性完成了四项运动：30 分钟 50%最大摄氧量（中等强度中等时间，MIMD），60 分钟 50%最大摄氧量（中等强度长时间，MILD），30 分钟 80%最大摄氧量（高强度中等时间，HIMD）和 80%最大摄氧量（时间达到 MIMD 的热量当量，HISD）。硫代巴比妥酸反应物质（TBARS）被测量为氧化应激的指标，肱动脉血流介导的扩张（FMD）被评估为内皮功能的指标。变量在基线、运动后即刻、1 小时和 2 小时测量。HIMD（14.2±2.5μmol/L）和 HISD（14.7±1.9μmol/L）TBARS 与 MIMD（11.8±1.5μmol/L）在运动后即刻不同。TBARS 从运动前到运动后即刻增加，HIMD（12.6±2.1 比 14.2±2.5μmol/L）和 HISD（12.3±2.8 比 14.7±1.9μmol/L）。MIMD（7.2±2.2%）和 HISD（7.6±2.7%）FMD 在运动后即刻均大于 HIMD（4.7±2.2%）。MIMD（5.0±2.5 比 7.2±2.2%）和 HISD（5.9±2.4 比 7.6±2.7%）FMD 从运动前到运动后即刻增加。这些数据表明，急性运动诱导的 TBARS 与运动强度有关，而 FMD 似乎在能量消耗相当于 30 分钟 50%最大摄氧量（VO2peak）后得到改善，无论强度或时间如何。
