DNA Database Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel.
Forensic Sci Int Genet. 2012 Jan;6(1):108-12. doi: 10.1016/j.fsigen.2011.03.010. Epub 2011 Apr 6.
During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be >22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus™ kit. To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGM(TM) (Applied Biosystem), Powerplex™ ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus™ profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGM(TM) and Powerplex™ ESX which also showed discordance in the calculated results between original SGM Plus™ designations and those obtained with the European new generation multiplexes.
在以色列警方 DNA 数据库的常规数据库样本分析过程中，使用 Applied Biosystems SGM Plus™试剂盒观察到一个梯外 D3S1358 等位基因，计算值为>22.1，延伸到相邻的 vWA 基因座。为了验证这个 D3S1358 长等位基因的大小，并确保它不是相邻基因座中三等位基因模式的一部分，使用三种欧洲新一代 STR 多重扩增试剂盒对样本进行了扩增：NGM(TM)（Applied Biosystem）、Powerplex™ ESX 和 ESI（Promega）。这些扩增的结果确定该变体是一个 22 等位基因。随后的测序证实了这一指定，并揭示了一个核苷酸多态性。另外 10 个 SGM Plus™ 分析的 D3S1358 等位基因大于 19 的样本，使用 NGM(TM) 和 Powerplex™ ESX 进行了重新分析，这些试剂盒的计算结果与原始 SGM Plus™ 指定结果以及与欧洲新一代多重扩增试剂盒获得的结果之间也存在不一致。
