Yim Su Youn, Lee You Jin, Lee Yoen Kyung, Jung Seung Eun, Kim Ji Ha, Kim Hak Jin, Son Beung Gu, Park Young Hoon, Lee Young Guen, Choi Young Whan, Hwang Dae Youn
Department of Laboratory Animal Resources, National Institute of Toxicological Research, Korean FDA, Seoul 122-704, Korea.
Mol Med Rep. 2009 Sep-Oct;2(5):725-32. doi: 10.3892/mmr_00000163.
Lignans isolated from Schisandria chinensis have been prescribed as anti-cancer and anti-hepatitis treatments in Chinese medicine. To investigate the applications of lignans isolated from Schisandria chinensis in hepatic carcinoma therapy, their apoptotic ability was screened using a cell proliferation assay. Compared to the other lignans, gomisin N induced high apoptotic levels in hepatic carcinoma. Cell morphology and flow cytometric analysis demonstrated that this lignan induced cell death at high concentrations, but did not induce any changes at low concentrations. In addition, the expression levels of Bcl-2 and Bax proteins, which are involved in the apoptotic pathway, were markedly increased in only the 320 µM-treated group compared to the vehicle and other concentration groups, while the expression level of p53 protein remained unchanged in this group. These results suggest that gomisin N is an anti-cancer drug candidate capable of inhibiting the proliferation and inducing the apoptosis of human hepatic carcinomas.
从五味子中分离出的木脂素在中国医学中已被用作抗癌和抗肝炎治疗药物。为了研究从五味子中分离出的木脂素在肝癌治疗中的应用，通过细胞增殖试验筛选了它们的凋亡能力。与其他木脂素相比，戈米辛N在肝癌中诱导了较高的凋亡水平。细胞形态学和流式细胞术分析表明，这种木脂素在高浓度下诱导细胞死亡，但在低浓度下未引起任何变化。此外，与载体组和其他浓度组相比，仅在320µM处理组中，参与凋亡途径的Bcl-2和Bax蛋白的表达水平显著增加，而该组中p53蛋白的表达水平保持不变。这些结果表明，戈米辛N是一种能够抑制人肝癌细胞增殖并诱导其凋亡的抗癌候选药物。
